{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T18:47:29Z","timestamp":1788202049449,"version":"build-2803163510"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"3","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,2,4]]},"abstract":"<jats:p>\n                    We show here sodium ion cycling between complex I from\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    and the F\n                    <jats:sub>1<\/jats:sub>\n                    F\n                    <jats:sub>0<\/jats:sub>\n                    ATP synthase from\n                    <jats:italic>Ilyobacter tartaricus<\/jats:italic>\n                    in a reconstituted proteoliposome system. In the course of NADH oxidation by complex I, an electrochemical sodium ion gradient was established and served as a driving force for the synthesis of ATP from ADP and phosphate. In the opposite direction, the \u0394\u03bc\u0303\n                    <jats:sub>\n                      Na\n                      <jats:sup>+<\/jats:sup>\n                    <\/jats:sub>\n                    generated by ATP hydrolysis could be coupled to NADH formation by reversed electron transfer from ubiquinol to NAD. For reverse electron transfer, a transmembrane voltage larger than 30 mV was obligatory. No NADH-driven proton transport into the lumen of proteoliposomes was detected. We conclude that Na\n                    <jats:sup>+<\/jats:sup>\n                    is used as the exclusive coupling ion by the enterobacterial complex I.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0237328100","type":"journal-article","created":{"date-parts":[[2003,2,4]],"date-time":"2003-02-04T12:24:41Z","timestamp":1044361481000},"page":"839-844","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":63,"title":["Sodium ion cycling mediates energy coupling between complex I and ATP synthase"],"prefix":"10.1073","volume":"100","author":[{"given":"Anja C.","family":"Gemperli","sequence":"first","affiliation":[{"name":"Mikrobiologisches Institut der Eidgen\u00f6ssischen Technischen Hochschule, Schmelzbergstrasse 7, CH-8092 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Dimroth","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut der 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