{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:26:04Z","timestamp":1787005564081,"version":"build-2736575974"},"reference-count":56,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,2,3]]},"abstract":"<jats:p>\n                    The antigenic component of a common Lyme disease vaccine is recombinant outer surface protein A (rOspA) of\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    (\n                    <jats:italic>Bb<\/jats:italic>\n                    ), the causative agent of Lyme disease. Coincidentally, patients with chronic, treatment-resistant Lyme arthritis develop an immune response against OspA, whereas those with acute Lyme disease usually do not. Treatment-resistant Lyme arthritis occurs in a subset of Lyme arthritis patients and is linked to HLA.DRB1*0401 (DR4) and related alleles. Recent work from our laboratory identified T cell crossreactivity between epitopes of OspA and lymphocyte function-associated antigen 1\u03b1\n                    <jats:sub>L<\/jats:sub>\n                    chain (LFA-1\u03b1\n                    <jats:sub>L<\/jats:sub>\n                    ) in these patients. We generated a form of rOspA, FTK-OspA, in which the LFA-1\u03b1\n                    <jats:sub>L<\/jats:sub>\n                    \/rOspA crossreactive T cell epitope was mutated to reduce the possible risk of autoimmunity in genetically susceptible individuals. FTK-OspA did not stimulate human or mouse DR4-restricted, WT-OspA-specific T cells, whereas it did stimulate antibody responses specific for WT-OspA that were similar to mice vaccinated WT-OspA. We show here that the protective efficacy of FTK-OspA is indistinguishable from that of WT-OspA in vaccination trials, as both C3H\/HeJ and BALB\/c FTK-OspA-vaccinated mice were protected from\n                    <jats:italic>Bb<\/jats:italic>\n                    infection. These data demonstrate that this rOspA-derived vaccine lacking the predicted cross-reactive T cell epitope, but retaining the capacity to elicit antibodies against infection, is effective in generating protective immunity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0305680101","type":"journal-article","created":{"date-parts":[[2004,2,3]],"date-time":"2004-02-03T12:47:24Z","timestamp":1075812444000},"page":"1303-1308","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":47,"title":["An effective second-generation outer surface protein A-derived Lyme vaccine that eliminates a potentially autoreactive T cell epitope"],"prefix":"10.1073","volume":"101","author":[{"given":"Theresa A.","family":"Willett","sequence":"first","affiliation":[{"name":"Department of Pathology, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111; and Center for Extracellular Matrix Biology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&amp;M University Health Science Center, Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbie L.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Pathology, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111; and Center for Extracellular Matrix Biology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&amp;M University Health Science Center, Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric L.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Pathology, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111; and Center for Extracellular Matrix Biology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&amp;M University Health Science Center, Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte T.","family":"Huber","sequence":"additional","affiliation":[{"name":"Department of Pathology, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111; and Center for Extracellular Matrix Biology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&amp;M University Health Science Center, Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,1,23]]},"reference":[{"key":"e_1_3_2_1_2","first-page":"1","volume":"49","year":"2000","unstructured":"Orloski, K. A., Hayes, E. B., Campbell, G. L. & Dennis, D. T. (2000) Morbid. Mortal. Wkly. Rep. CDC Surveill. Summ. 49, 1\u201311.","journal-title":"Morbid. Mortal. Wkly. Rep. CDC Surveill. 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