{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T16:39:46Z","timestamp":1793723986669,"version":"4.6.1"},"reference-count":44,"publisher":"National Academy of Sciences","issue":"9","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,3,2]]},"abstract":"<jats:p>\n                    Environmentally responsive synthesis of surface proteins represents a hallmark of the infectious cycle of the Lyme disease agent,\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    . Here we created and analyzed a\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    mutant lacking outer-surface protein C (OspC), an abundant Osp that spirochetes normally synthesize in the tick vector during the blood meal and down-regulate after transmission to the mammal. We demonstrate that\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    strictly requires OspC to infect mice but not to localize or migrate appropriately in the tick. The induction of a spirochetal virulence factor preceding the time and host in which it is required demonstrates a developmental sequence for transmission of this arthropod-borne pathogen.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0306845101","type":"journal-article","created":{"date-parts":[[2004,3,2]],"date-time":"2004-03-02T21:33:11Z","timestamp":1078263191000},"page":"3142-3147","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":374,"title":["Outer-surface protein C of the Lyme disease spirochete: A protein induced in ticks for infection of mammals"],"prefix":"10.1073","volume":"101","author":[{"given":"Dorothee","family":"Grimm","sequence":"first","affiliation":[{"name":"Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840; and Department of Molecular Biology, University of Wyoming, Laramie, WY 82071"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kit","family":"Tilly","sequence":"additional","affiliation":[{"name":"Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840; 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