{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:24:57Z","timestamp":1781108697792,"version":"3.54.1"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,1,27]]},"abstract":"<jats:p>\n                    Within the B cell antigen receptor (BCR), the cytoplasmic tails of both Ig\u03b1 and Ig\u03b2 are required for normal B cell development and maturation. To dissect the mechanisms by which each tail contributes to development\n                    <jats:italic>in vivo<\/jats:italic>\n                    , Ig\u03b2\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice were reconstituted with retroviruses encoding either wild-type Ig\u03b2, an Ig\u03b2 molecule lacking a cytoplasmic tail (Ig\u03b2\n                    <jats:sup>\u0394C<\/jats:sup>\n                    ) or one in which the cytoplasmic tail was derived from Ig\u03b1 (Ig\u03b2\n                    <jats:sup>C\u03b1<\/jats:sup>\n                    ). All constructs rescued B cell development and generated immature B cell populations in the bone marrow with similar expression levels of both Ig\u03b2 and membrane-bound IgM. In the periphery, receptor-surface density was inversely proportional to the number of Ig\u03b1 tails in the BCR. Although peripheral-surface-receptor levels differed, splenic B cells expressing either Ig\u03b2 or Ig\u03b2\n                    <jats:sup>C\u03b1<\/jats:sup>\n                    responded similarly to stimulation through the BCR. Analysis of membrane-bound IgM and Ig\u03b2 expression revealed that peripheral-receptor expression was primarily determined by positive selection between the bone marrow and peripheral immature B cell populations. These data indicate that B cells are selected into the periphery on the basis of a common level of antigen responsiveness.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0307040101","type":"journal-article","created":{"date-parts":[[2004,1,27]],"date-time":"2004-01-27T13:23:16Z","timestamp":1075209796000},"page":"1027-1032","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":6,"title":["Selection of B lymphocytes in the periphery is determined by the functional capacity of the B cell antigen receptor"],"prefix":"10.1073","volume":"101","author":[{"given":"Leo D.","family":"Wang","sequence":"first","affiliation":[{"name":"Section of Rheumatology and Committee on Immunology, Biological Sciences Division and Pritzker School of Medicine, University of Chicago, Chicago, IL 60637; and Cell Biology Group, Department of Zoology, University of British Columbia, Vancouver, BC, Canada V6T 1Z4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared","family":"Lopes","sequence":"additional","affiliation":[{"name":"Section of Rheumatology and Committee on Immunology, Biological Sciences Division and Pritzker School of Medicine, University of Chicago, Chicago, IL 60637; and Cell Biology Group, Department of Zoology, University of British Columbia, Vancouver, BC, Canada V6T 1Z4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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