{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:28:41Z","timestamp":1791329321623,"version":"4.3.1"},"reference-count":45,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,4,6]]},"abstract":"<jats:p>\n                    <jats:italic>Sinorhizobium meliloti<\/jats:italic>\n                    , a legume symbiont, and\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    , a phylogenetically related mammalian pathogen, both require the bacterial-encoded BacA protein to establish chronic intracellular infections in their respective hosts. We found that the bacterial BacA proteins share sequence similarity with a family of eukaryotic peroxisomal-membrane proteins, including the human adrenoleukodystrophy protein, required for the efficient transport of very-long-chain fatty acids out of the cytoplasm. This insight, along with the increased sensitivity of BacA-deficient mutants to detergents and cell envelope-disrupting agents, led us to discover that BacA affects the very-long-chain fatty acid (27-OHC28:0 and 29-OHC30:0) content of both\n                    <jats:italic>Sinorhizobium<\/jats:italic>\n                    and\n                    <jats:italic>Brucella<\/jats:italic>\n                    lipid A. We discuss models for how BacA function affects the lipid-A fatty-acid content and why this activity could be important for the establishment of chronic intracellular infections.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0307137101","type":"journal-article","created":{"date-parts":[[2004,3,25]],"date-time":"2004-03-25T08:18:00Z","timestamp":1080202680000},"page":"5012-5017","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":105,"title":["Similarity to peroxisomal-membrane protein family reveals that\n                    <i>Sinorhizobium<\/i>\n                    and\n                    <i>Brucella<\/i>\n                    BacA affect lipid-A fatty acids"],"prefix":"10.1073","volume":"101","author":[{"given":"Gail P.","family":"Ferguson","sequence":"first","affiliation":[{"name":"Biology Department, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139; Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602; and Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anup","family":"Datta","sequence":"additional","affiliation":[{"name":"Biology Department, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139; Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602; and Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Baumgartner","sequence":"additional","affiliation":[{"name":"Biology Department, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139; Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602; and Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"R. Martin","family":"Roop","sequence":"additional","affiliation":[{"name":"Biology Department, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139; Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602; and Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russ W.","family":"Carlson","sequence":"additional","affiliation":[{"name":"Biology Department, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139; Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602; and Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham C.","family":"Walker","sequence":"additional","affiliation":[{"name":"Biology Department, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139; Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602; and Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,3,24]]},"reference":[{"key":"e_1_3_2_1_2","first-page":"51","volume":"24","year":"1998","unstructured":"Niner, B. 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