{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:42:04Z","timestamp":1789990924799,"version":"4.0.1"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,2,10]]},"abstract":"<jats:p>\n                    In mice, ovariectomy accelerates the progression of the end-stage renal disease glomerulosclerosis. In women, the incidence of this disease increases after menopause, and estrogen alters its progression. Polymorphisms in the human estrogen receptor \u03b1 (ER\u03b1) gene have been suggested to constitute a genetic predisposition for lupus nephritis. Here we show that by 1 year of age, mice lacking ER\u03b1 (ER\u03b1\n                    <jats:sup>-\/-<\/jats:sup>\n                    ) but not those lacking ER\u03b2 (ER\u03b2\n                    <jats:sup>-\/-<\/jats:sup>\n                    ) exhibit immune complex-type glomerulonephritis, proteinuria, and destruction of tubular cells with severe infiltration of B lymphocytes in the kidney and the presence of anti-DNA antibodies in serum. No gender difference occurred in the incidence or severity of these symptoms. However, in female but not in male ER\u03b1\n                    <jats:sup>-\/-<\/jats:sup>\n                    mice there were elevated serum levels of IgG3. Other prominent features of these mice were (\n                    <jats:italic>i<\/jats:italic>\n                    ) spontaneous formation of germinal centers in the spleen in the absence of antigen challenge and (\n                    <jats:italic>ii<\/jats:italic>\n                    ) infiltration of plasma cells in the kidney and plasmacytosis in the spleen. Immunohistochemistry indicated a selective expression of ER\u03b1 protein in the germinal centers but not in the follicular mantle zone of murine spleens and human tonsils. Our results indicate that ER\u03b1 has indispensable functions in the kidney and in germinal centers, and that defective ER\u03b1 signaling results in glomerulonephritis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0307915100","type":"journal-article","created":{"date-parts":[[2004,2,10]],"date-time":"2004-02-10T13:32:51Z","timestamp":1076419971000},"page":"1720-1724","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":71,"title":["Autoimmune glomerulonephritis with spontaneous formation of splenic germinal centers in mice lacking the\n                    <i>estrogen receptor alpha<\/i>\n                    gene"],"prefix":"10.1073","volume":"101","author":[{"given":"Gil-Jin","family":"Shim","sequence":"first","affiliation":[{"name":"Department of Biosciences at NOVUM, Karolinska Institute, NOVUM, S-14157 Huddinge, Sweden; Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Solna, Stockholm, Sweden; and Department of Medical Nutrition, Karolinska Institute, NOVUM, S-141 86 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lor\u00e1nd Levente","family":"Kis","sequence":"additional","affiliation":[{"name":"Department of Biosciences at NOVUM, Karolinska Institute, NOVUM, S-14157 Huddinge, Sweden; Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Solna, Stockholm, Sweden; and Department of Medical Nutrition, Karolinska Institute, NOVUM, S-141 86 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Warner","sequence":"additional","affiliation":[{"name":"Department of Biosciences at NOVUM, Karolinska Institute, NOVUM, S-14157 Huddinge, Sweden; Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Solna, Stockholm, Sweden; and Department of Medical Nutrition, Karolinska Institute, NOVUM, S-141 86 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan-\u00c5ke","family":"Gustafsson","sequence":"additional","affiliation":[{"name":"Department of Biosciences at NOVUM, Karolinska Institute, NOVUM, S-14157 Huddinge, Sweden; Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Solna, Stockholm, Sweden; and Department of Medical Nutrition, Karolinska Institute, NOVUM, S-141 86 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,1,26]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V122241"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002-220728"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000049039"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1681.2002.03603.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)61134-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64277-0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.5.2271"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/smim.1996.0022"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.8.11.8070632"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.4049\/jimmunol.142.5.1470","volume":"142","year":"1989","unstructured":"Carteron, N. 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