{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:50:05Z","timestamp":1781110205511,"version":"3.54.1"},"reference-count":37,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,2,24]]},"abstract":"<jats:p>\n                    CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD45RO\n                    <jats:sup>+<\/jats:sup>\n                    T cells are the major latent viral reservoir in HIV-infected individuals and hence a major obstacle in curing the disease. An anti-CD45RO immunotoxin (IT) can decrease the number of both productively and latently infected CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells obtained from HIV-infected individuals with detectable viremia. In this study, we determined whether this IT could also kill latently infected replication-competent CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells obtained from infected individuals without detectable plasma viremia. Our results demonstrate that\n                    <jats:italic>ex vivo<\/jats:italic>\n                    treatment with the anti-CD45RO IT significantly reduced the frequency of these cells. In contrast, the IT had only a modest effect on the cytomegalalovirus-specific memory responses of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. These results suggest that purging latent cells from infected individuals on highly active antiretroviral therapy with the anti-CD45RO IT might reduce the HIV latent reservoir without seriously compromising CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell memory responses.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0308381100","type":"journal-article","created":{"date-parts":[[2004,2,24]],"date-time":"2004-02-24T23:03:03Z","timestamp":1077663783000},"page":"2494-2499","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":14,"title":["An anti-CD45RO immunotoxin kills HIV-latently infected cells from individuals on HAART with little effect on CD8 memory"],"prefix":"10.1073","volume":"101","author":[{"given":"J.","family":"Saavedra-Lozano","sequence":"first","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Cao","sequence":"additional","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Callison","sequence":"additional","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Sarode","sequence":"additional","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Sodora","sequence":"additional","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Edgar","sequence":"additional","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hatfield","sequence":"additional","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Picker","sequence":"additional","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Peterson","sequence":"additional","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Ramilo","sequence":"additional","affiliation":[{"name":"Cancer Immunobiology Center, Departments of Pediatrics, Pathology, Microbiology, and Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; and Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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