{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:36:24Z","timestamp":1781105784604,"version":"3.54.1"},"reference-count":20,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,3,9]]},"abstract":"<jats:p>\n                    The biosynthesis\n                    <jats:italic>in vitro<\/jats:italic>\n                    of phosphorylcholine oligosaccharides in\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    has been investigated. Here we show that extracts of\n                    <jats:italic>C. elegans<\/jats:italic>\n                    ' microsomes transfer phosphorylcholine from L-\u03b1-dipalmitoyl phosphatidylcholine to hybrid and complex type N-linked oligosaccharides containing mannose residues disubstituted with\n                    <jats:italic>N<\/jats:italic>\n                    -acetylglucosamine. The reaction products are consistent with structures reported for\n                    <jats:italic>C. elegans<\/jats:italic>\n                    as well those found in the filarial nematodes\n                    <jats:italic>Acanthocheilonema viteae<\/jats:italic>\n                    ,\n                    <jats:italic>Onchocerca volvulus<\/jats:italic>\n                    , and\n                    <jats:italic>Brugia malayi<\/jats:italic>\n                    , strongly supporting the concept that the phosphorylcholine oligosaccharide biosynthetic enzymes are conserved in this group of organisms. Because it is thought that phosphorylcholine substitution of oligosaccharides modulates host immune response in filarial infections, this\n                    <jats:italic>in vitro<\/jats:italic>\n                    system may help in gaining an understanding of the basis for this response.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0400384101","type":"journal-article","created":{"date-parts":[[2004,3,11]],"date-time":"2004-03-11T15:20:15Z","timestamp":1079018415000},"page":"3404-3408","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":33,"title":["Biosynthesis\n                    <i>in vitro<\/i>\n                    of\n                    <i>Caenorhabditis elegans<\/i>\n                    phosphorylcholine oligosaccharides"],"prefix":"10.1073","volume":"101","author":[{"given":"John F.","family":"Cipollo","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, 715 Albany Street, Boston, MA 02118-2526; and Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118-2526"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Awad","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, 715 Albany Street, Boston, MA 02118-2526; and Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118-2526"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine E.","family":"Costello","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, 715 Albany Street, Boston, MA 02118-2526; and Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118-2526"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillips W.","family":"Robbins","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, 715 Albany Street, Boston, MA 02118-2526; and Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118-2526"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos B.","family":"Hirschberg","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, 715 Albany Street, Boston, MA 02118-2526; and Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118-2526"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,3]]},"reference":[{"key":"e_1_3_2_1_2","unstructured":"U.S. Congress Office of Technology Assessment (1985) Status of Biomedical Research and Related Technology for Tropical Diseases OTA-H-258 (U.S. Government Printing Office Washington D.C.) 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