{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:57:36Z","timestamp":1791338256440,"version":"4.3.1"},"reference-count":23,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,3,30]]},"abstract":"<jats:p>\n                    The Wave proteins are major activators of the Arp2\/3 complex. The ubiquitous Wave-2 is required for actin polymerization at the leading edge of migrating cells. Here we purify Wave-2 from HeLa cells. Five proteins, Sra, Nap, Wave-2, Abi, and Hspc, are copurified, indicating that they form a tight complex. These proteins are only present in the complexed form, with the exception of Hspc, which displays a free pool. We reconstitute the Wave-2 complex by cotranslating\n                    <jats:italic>in vitro<\/jats:italic>\n                    the five subunits and use this system together with specific immunoprecipitations to study the molecular architecture of the complex. The complex is organized around a core of Nap and Abi. Sra is a peripheral subunit recruited on the Nap side, whereas the Wave and Hspc subunits are recruited on the Abi side of the core.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0400628101","type":"journal-article","created":{"date-parts":[[2004,3,19]],"date-time":"2004-03-19T20:13:08Z","timestamp":1079727188000},"page":"4379-4383","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":222,"title":["Purification and architecture of the ubiquitous Wave complex"],"prefix":"10.1073","volume":"101","author":[{"given":"Alexis","family":"Gautreau","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-yi H.","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanno","family":"Steen","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven P.","family":"Gygi","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc W.","family":"Kirschner","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,3,19]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00120-X"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kve197"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1001-897"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02239-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg350"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01770"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01148"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.23.6932"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/35047107"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00859"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.3.1720"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.1.291"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0264"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0894"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.21.2583"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb886"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.22.13681"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00580-3"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.10.005"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200303023"},{"key":"e_1_3_2_21_2","first-page":"4427","volume":"130","year":"2003","unstructured":"Bogdan, S. & Klambt, C. (2003) Development (Cambridge, U.K.) 130, 4427\u20134437.","journal-title":"Development (Cambridge, U.K.)"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3739"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1066333"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0400628101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T09:41:41Z","timestamp":1649756501000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0400628101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,3,19]]},"references-count":23,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2004,3,30]]}},"alternative-id":["10.1073\/pnas.0400628101"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0400628101","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,3,19]]},"assertion":[{"value":"2004-03-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}