{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T17:01:35Z","timestamp":1781110895735,"version":"3.54.1"},"reference-count":20,"publisher":"National Academy of Sciences","issue":"18","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,5,4]]},"abstract":"<jats:p>\n                    Since the discovery of the active metabolite of vitamin D, i.e., 1\u03b1,25-dihydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    , there has been a continuous effort to synthesize analogs able to carry out many of the functions of the native hormone without raising serum calcium concentration. The present report provides a series of previously undescribed analogs wherein this goal is realized. We have prepared 2-methylene-19-nor-1\u03b1-hydroxyvitamin D analogs of 1,25-(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    that possess only two to four carbons of the side chain without a hydroxyl thereon. Compared to 1,25-(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    , these analogs are slightly less active in binding to the vitamin D receptor, in causing HL-60 differentiation, and are slightly less active in\n                    <jats:italic>in vitro<\/jats:italic>\n                    transcription assays using the 24-hydroxylase promoter attached to a luciferase reporter gene. Of considerable importance is that these analogs, given to rats at daily doses of up to 70 \u03bcg\/kg of body weight per day, are either unable or only slightly able to raise serum calcium concentration but are nevertheless able to suppress parathyroid hormone levels in plasma up to 100% and induce 24-hydroxylase mRNA in skin. Because of their ability to act\n                    <jats:italic>in vivo<\/jats:italic>\n                    without raising serum calcium levels, they may be of considerable interest for the systemic treatment of diseases such as psoriasis, cancer, and secondary hyperparathyroidism of renal failure, where a rise in serum calcium is undesirable.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0401656101","type":"journal-article","created":{"date-parts":[[2004,4,26]],"date-time":"2004-04-26T20:54:03Z","timestamp":1083012843000},"page":"6900-6904","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":54,"title":["Biologically active noncalcemic analogs of 1\u03b1,25-dihydroxyvitamin D with an abbreviated side chain containing no hydroxyl"],"prefix":"10.1073","volume":"101","author":[{"given":"Lori A.","family":"Plum","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin, Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean M.","family":"Prahl","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin, Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin, Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafal R.","family":"Sicinski","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin, Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumithra","family":"Gowlugari","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin, Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Clagett-Dame","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin, Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector F.","family":"DeLuca","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin, Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,4,26]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.4.1193"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0272-6386(95)90455-7"},{"key":"e_1_3_2_3_2","first-page":"1071","volume":"63","year":"1997","unstructured":"Kubodera, N., Sato, K. & Nishii, Y. 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