{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:00:54Z","timestamp":1790751654472,"version":"4.1.0"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"29","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,7,20]]},"abstract":"<jats:p>\n                    ACT [activator of cAMP-responsive element modulator (CREM) in testis] is a LIM-only protein that interacts with transcription factor CREM in postmeiotic male germ cells and enhances CREM-dependent transcription. CREM regulates many crucial genes required for spermatid maturation, and targeted mutation of the\n                    <jats:italic>Crem<\/jats:italic>\n                    gene in the mouse germ-line blocks spermatogenesis. Here we report the phenotype of mice in which targeted disruption of the\n                    <jats:italic>act<\/jats:italic>\n                    gene was obtained by homologous recombination. Whereas the seminiferous tubules of the\n                    <jats:italic>act<\/jats:italic>\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice contain all of the developmental stages of germ cells and the mice are fertile, the amount of mature sperm in the epididymis is drastically reduced. The residual sperm display severe abnormalities, including fully folded tails and aberrant head shapes. These results indicate that numerous postmeiotic genes under CREM control require the coactivator function of ACT. Thus, the fine-tuning of sperm development is achieved by the coordinated action of two transcriptional regulators.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0401947101","type":"journal-article","created":{"date-parts":[[2004,7,9]],"date-time":"2004-07-09T21:07:34Z","timestamp":1089407254000},"page":"10620-10625","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":67,"title":["Abnormal sperm in mice with targeted deletion of the\n                    <i>act<\/i>\n                    (activator of cAMP-responsive element modulator in testis) gene"],"prefix":"10.1073","volume":"101","author":[{"given":"Noora","family":"Kotaja","sequence":"first","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, B.P. 10142, 67404 Illkirch-Strasbourg, France; and Centre for Reproductive Medicine, University Hospital, Dutch-Speaking Brussels Free University, Laarbeeklaan 101, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"De Cesare","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, B.P. 10142, 67404 Illkirch-Strasbourg, France; and Centre for Reproductive Medicine, University Hospital, Dutch-Speaking Brussels Free University, Laarbeeklaan 101, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betina","family":"Macho","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, B.P. 10142, 67404 Illkirch-Strasbourg, France; and Centre for Reproductive Medicine, University Hospital, Dutch-Speaking Brussels Free University, Laarbeeklaan 101, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Monaco","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, B.P. 10142, 67404 Illkirch-Strasbourg, France; and Centre for Reproductive Medicine, University Hospital, Dutch-Speaking Brussels Free University, Laarbeeklaan 101, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Brancorsini","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, B.P. 10142, 67404 Illkirch-Strasbourg, France; and Centre for Reproductive Medicine, University Hospital, Dutch-Speaking Brussels Free University, Laarbeeklaan 101, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Goossens","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, B.P. 10142, 67404 Illkirch-Strasbourg, France; and Centre for Reproductive Medicine, University Hospital, Dutch-Speaking Brussels Free University, Laarbeeklaan 101, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman","family":"Tournaye","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, B.P. 10142, 67404 Illkirch-Strasbourg, France; and Centre for Reproductive Medicine, University Hospital, Dutch-Speaking Brussels Free University, Laarbeeklaan 101, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Gansmuller","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, B.P. 10142, 67404 Illkirch-Strasbourg, France; and Centre for Reproductive Medicine, University Hospital, Dutch-Speaking Brussels Free University, Laarbeeklaan 101, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Sassone-Corsi","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, B.P. 10142, 67404 Illkirch-Strasbourg, France; and Centre for Reproductive Medicine, University Hospital, Dutch-Speaking Brussels Free University, Laarbeeklaan 101, B-1090 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,7,9]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000990303"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod44.4.569"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10311"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10320"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1070963"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/355080a0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.1998.0200"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/380159a0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/380162a0"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/me.2003-0280"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/18237"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077265"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.22.8613-8622.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90503-Q"},{"key":"e_1_3_2_15_2","first-page":"1502","volume":"7","year":"1993","unstructured":"Delmas, V., van der Hoorn, F., Mellstrom, B., Jegou, B. & Sassone-Corsi, P. 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