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The possibility to systematically incorporate foreign genes into the genome of RV and produce a recombinant virus allows us to examine whether the immunogenicity of foreign antigens can be enhanced by incorporation into the RV RNP structure. To test this hypothesis we constructed a recombinant RV expressing a RV N-GFP fusion protein. The chimeric N-GFP fusion protein was efficiently expressed and incorporated into RV RNP and virions. Moreover, the recombinant RNP induces a strong humoral immune response against GFP in mice. In contrast, mice inoculated with GFP alone or a combination of wild-type RV RNPs and GFP did not trigger any GFP-specific humoral responses using the same immunization schedule. These data indicate the usefulness of RV-based vectors as killed vaccines against other infectious diseases.<\/jats:p>","DOI":"10.1073\/pnas.0403060101","type":"journal-article","created":{"date-parts":[[2004,6,15]],"date-time":"2004-06-15T14:30:48Z","timestamp":1087309848000},"page":"9405-9410","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":32,"title":["Rabies virus nucleoprotein as a carrier for foreign antigens"],"prefix":"10.1073","volume":"101","author":[{"given":"Martin L.","family":"Koser","sequence":"first","affiliation":[{"name":"Departments of Biochemistry and Molecular Pharmacology and Microbiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107-5541"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"McGettigan","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Pharmacology and Microbiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107-5541"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene S.","family":"Tan","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Pharmacology and Microbiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107-5541"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Ellen","family":"Smith","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Pharmacology and Microbiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107-5541"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilary","family":"Koprowski","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Pharmacology and Microbiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107-5541"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Dietzschold","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Pharmacology and Microbiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107-5541"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias J.","family":"Schnell","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Pharmacology and Microbiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107-5541"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,6,14]]},"reference":[{"key":"e_1_3_2_1_2","first-page":"1121","volume":"1","year":"1996","unstructured":"Wagner, R. 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