{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:35:01Z","timestamp":1781105701056,"version":"3.54.1"},"reference-count":42,"publisher":"National Academy of Sciences","issue":"33","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,8,17]]},"abstract":"<jats:p>\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    myosin IC (AMIC) is a single-headed myosin comprised of one heavy chain (129 kDa) and one light chain (17 kDa). The heavy chain has head, neck (light chain-binding), and tail domains. The tail consists of four subdomains: a basic region (BR) (23 kDa) and two Gly\/Pro\/Ala-rich (GPA) regions, GPA1 (6 kDa) and GPA2 (15 kDa), flanking an Src homology 3 region (6 kDa). Although the AMIC head is similar in sequence, structure, and function (ATPase motor) to other myosin heads, the organization of the tail has been less clear as has its function beyond an assumed role in binding interaction partners, e.g., the BR has a membrane affinity and the GPA components bind F-actin in an ATP-independent manner. To investigate the spatial arrangement of subdomains in the tail, we have used cryo-electron microscopy and image reconstruction to compare actin filaments decorated with WT AMIC and tail-truncated mutants of various lengths. The BR forms an oval-shaped feature, \u224840 \u00c5 long, that diverges obliquely from the head, extending azimuthally around the actin filament and toward its barbed end. GPA2 and GPA1 are located together on the inner (actin-proximal) side of the tail, close enough to act in concert in binding the same or another actin filament. The outer face of the BR is strategically exposed for membrane or vesicle binding.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0404835101","type":"journal-article","created":{"date-parts":[[2004,8,9]],"date-time":"2004-08-09T20:16:45Z","timestamp":1092082605000},"page":"12189-12194","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":7,"title":["Subdomain organization of the\n                    <i>Acanthamoeba<\/i>\n                    myosin IC tail from cryo-electron microscopy"],"prefix":"10.1073","volume":"101","author":[{"given":"Takashi","family":"Ishikawa","sequence":"first","affiliation":[{"name":"Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, and Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naiqian","family":"Cheng","sequence":"additional","affiliation":[{"name":"Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, and Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiong","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, and Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward D.","family":"Korn","sequence":"additional","affiliation":[{"name":"Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, and Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alasdair C.","family":"Steven","sequence":"additional","affiliation":[{"name":"Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal, and Skin Diseases, and Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,8,9]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"crossref","unstructured":"Sellers J. 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