{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:44:31Z","timestamp":1790858671111,"version":"4.1.0"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:p>\n                    During early mouse embryogenesis, each laminin (Lm) chain of the first described Lm, a heterotrimer of \u03b11, \u03b21, and \u03b31 chains (Lm-1), is essential for basement membrane (BM) assembly, which is required for pregastrulation development. Individual domains may have other functions, not necessarily structural. The cell binding C terminus of Lm \u03b11 chain contains five Lm globular (LG) domains.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , \u03b11LG1\u20133 domains bind integrins, and \u03b11LG4 binds dystroglycan, heparin, and sulfatides. A prevailing hypothesis is that \u03b11LG4 is crucial as a structural domain for BM assembly, whereas integrin-binding sites conduct signaling. The\n                    <jats:italic>in vivo<\/jats:italic>\n                    role of \u03b11LG4\u20135 (also called E3) has not been studied. Mice lacking \u03b11LG4\u20135 were therefore made. Null embryos implanted, but presumptive epiblast cells failed to polarize and did not survive past day 6.5. BM components including truncated Lm \u03b11 were detected in Reichert's membrane. Surprisingly, embryonic BM assembly between visceral endoderm and stem cells was normal in null embryos and in embryoid bodies of \u03b11LG4\u20135-null embryonic stem cells. Yet, stem cells could not develop into polarized epiblast cells. Thus, \u03b11LG4\u20135 provides vital signals for the conversion of stem cells to polarized epithelium.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0405095102","type":"journal-article","created":{"date-parts":[[2005,1,24]],"date-time":"2005-01-24T20:26:07Z","timestamp":1106598367000},"page":"1502-1506","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":59,"title":["Laminin \u03b11 globular domains 4\u20135 induce fetal development but are not vital for embryonic basement membrane assembly"],"prefix":"10.1073","volume":"102","author":[{"given":"Susanne","family":"Sch\u00e9ele","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology, Lund University, 22184 Lund, Sweden; Department of Animal Physiology, Uppsala University, 751 05 Uppsala, Sweden; Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel; and Laboratory of Protein Chemistry, Max Planck Institute for Biochemistry, Martinsried 82152, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mats","family":"Falk","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Lund University, 22184 Lund, Sweden; Department of Animal Physiology, Uppsala University, 751 05 Uppsala, Sweden; Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel; and Laboratory of Protein Chemistry, Max Planck Institute for Biochemistry, Martinsried 82152, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahnders","family":"Franz\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Lund University, 22184 Lund, Sweden; Department of Animal Physiology, Uppsala University, 751 05 Uppsala, Sweden; Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel; and Laboratory of Protein Chemistry, Max Planck Institute for Biochemistry, Martinsried 82152, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredrik","family":"Ellin","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Lund University, 22184 Lund, Sweden; Department of Animal Physiology, Uppsala University, 751 05 Uppsala, Sweden; Center for Genomics and Bioinformatics, Karolinska Institutet, SE-171 77 Stockholm, Sweden; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel; and Laboratory of Protein Chemistry, Max Planck Institute for Biochemistry, Martinsried 82152, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Ferletta","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Lund University, 22184 Lund, Sweden; Department of Animal Physiology, Uppsala University, 751 05 Uppsala, Sweden; 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