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E\n                    <jats:sub>2<\/jats:sub>\n                    treatment resulted in a rise in anti-DNA serum titers and in Ig deposition in renal glomeruli. ELISPOT analysis confirmed a significant increase in the number of high-affinity anti-DNA antibody-secreting B cells in the spleens of E\n                    <jats:sub>2<\/jats:sub>\n                    -treated mice. Hybridomas generated from E\n                    <jats:sub>2<\/jats:sub>\n                    -treated mice express high-affinity, unmutated anti-DNA antibodies, indicating that na\u00efve B cells that are normally deleted or anergized are rescued from tolerance induction. Finally, immunohistochemical studies revealed increased Bcl-2 expression in splenic B cells of E\n                    <jats:sub>2<\/jats:sub>\n                    -treated mice. These data demonstrate that estrogen interferes with tolerance induction of na\u00efve autoreactive B cells and that the presence of these B cells in the periphery is associated with up-regulation of Bcl-2.\n                  <\/jats:p>","DOI":"10.1073\/pnas.040577497","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:38:21Z","timestamp":1027679901000},"page":"2703-2708","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":220,"title":["Estrogen up-regulates Bcl-2 and blocks tolerance induction of na\u00efve B cells"],"prefix":"10.1073","volume":"97","author":[{"given":"Margaret S.","family":"Bynoe","sequence":"first","affiliation":[{"name":"Departments of Microbiology and Immunology and Medicine, Albert Einstein College of Medicine, Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine 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