{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:17:14Z","timestamp":1791220634574,"version":"4.1.0"},"reference-count":26,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,4,5]]},"abstract":"<jats:p>\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    is generally viewed as an extracellular pathogen. We have analyzed the tropism of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    clinical isolates in a gnotobiotic transgenic mouse model of human chronic atrophic gastritis, a preneoplastic condition. These mice lack acid-producing parietal cells and have an amplified population of dividing gastric epithelial progenitors (GEPs) that express NeuAc\u03b12,3Gal\u03b21,4-glycans recognized by\n                    <jats:italic>H. pylori<\/jats:italic>\n                    adhesins. Scanning confocal and transmission electron microscopic studies of stomachs that had been colonized for 1 month or 1 year revealed intracellular bacterial collections (IBCs) in a small subset of multi- and oligopotential epithelial progenitors. Transmission electron microscopic and multilabel immunohistochemical analyses disclosed bacteria with several morphotypes, including spiral-shaped, in the cytoplasm and endosomes. Several stages in IBC evolution were documented, from a few solitary bacteria to consolidated populations in dividing and nondividing GEPs, to microorganisms traversing breaches in the GEP plasma cell membrane. IBC formation was not a unique feature of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    strains isolated from patients with chronic atrophic gastritis. The notion that adult mammalian epithelial progenitors can function as a repository for\n                    <jats:italic>H. pylori<\/jats:italic>\n                    broadens the view of host habitats available to this and perhaps other pathogens.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0407657102","type":"journal-article","created":{"date-parts":[[2005,3,28]],"date-time":"2005-03-28T20:14:19Z","timestamp":1112040859000},"page":"5186-5191","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":96,"title":["Intracellular\n                    <i>Helicobacter pylori<\/i>\n                    in gastric epithelial progenitors"],"prefix":"10.1073","volume":"102","author":[{"given":"Jung D.","family":"Oh","sequence":"first","affiliation":[{"name":"Center for Genome Sciences and Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63108; and Department of Anatomy, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain 17666, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherif M.","family":"Karam","sequence":"additional","affiliation":[{"name":"Center for Genome Sciences and Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63108; and Department of Anatomy, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain 17666, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey I.","family":"Gordon","sequence":"additional","affiliation":[{"name":"Center for Genome Sciences and Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63108; and Department of Anatomy, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain 17666, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,3,28]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.1.149"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.15.8359"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069076"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0277-5379(88)90203-9"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djh057"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/108.5.504"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5964-5971.1998"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(03)00020-8"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.2000.00023.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00222.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1084550"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308125100"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.7.3671"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80454-2"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0580-9517(02)31030-4"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203613200"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3925"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0230380100"},{"key":"e_1_3_2_19_2","first-page":"G1209","volume":"272","year":"1997","unstructured":"Karam, S. M., Li, Q. & Gordon, J. I. (1997) Am. J. Physiol. 272, G1209\u20131220.9176232","journal-title":"Am. J. Physiol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192574799"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2108-2120.2002"},{"key":"e_1_3_2_22_2","first-page":"181","volume":"7","year":"2004","unstructured":"Alonso, A. & Garcia-del Portillo, F. (2004) Int. Microbiol. 7, 181\u2013191.15492932","journal-title":"Int. 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