{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T07:03:22Z","timestamp":1791356602326,"version":"4.3.1"},"reference-count":25,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,3,8]]},"abstract":"<jats:p>\n                    The important role that cilia and flagella play in human disease creates an urgent need to identify genes involved in ciliary assembly and function. The strong and specific induction of flagellar-coding genes during flagellar regeneration in\n                    <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>\n                    suggests that transcriptional profiling of such cells would reveal new flagella-related genes. We have conducted a genome-wide analysis of RNA transcript levels during flagellar regeneration in\n                    <jats:italic>Chlamydomonas<\/jats:italic>\n                    by using maskless photolithography method-produced DNA oligonucleotide microarrays with unique probe sequences for all exons of the 19,803 predicted genes. This analysis represents previously uncharacterized whole-genome transcriptional activity profiling study in this important model organism. Analysis of strongly induced genes reveals a large set of known flagellar components and also identifies a number of important disease-related proteins as being involved with cilia and flagella, including the zebrafish polycystic kidney genes\n                    <jats:italic>Qilin<\/jats:italic>\n                    ,\n                    <jats:italic>Reptin<\/jats:italic>\n                    , and\n                    <jats:italic>Pontin<\/jats:italic>\n                    , as well as the testis-expressed tubby-like protein TULP2.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0408358102","type":"journal-article","created":{"date-parts":[[2005,2,28]],"date-time":"2005-02-28T20:24:54Z","timestamp":1109622294000},"page":"3703-3707","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":210,"title":["Genome-wide transcriptional analysis of flagellar regeneration in\n                    <i>Chlamydomonas reinhardtii<\/i>\n                    identifies orthologs of ciliary disease genes"],"prefix":"10.1073","volume":"102","author":[{"given":"Viktor","family":"Stolc","sequence":"first","affiliation":[{"name":"Genome Research Facility, National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; Eloret Corporation at National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143; and Systemix Institute, Cupertino, CA 95014"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj Pratim","family":"Samanta","sequence":"additional","affiliation":[{"name":"Genome Research Facility, National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; Eloret Corporation at National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143; and Systemix Institute, Cupertino, CA 95014"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waraporn","family":"Tongprasit","sequence":"additional","affiliation":[{"name":"Genome Research Facility, National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; Eloret Corporation at National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143; and Systemix Institute, Cupertino, CA 95014"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wallace F.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Genome Research Facility, National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; Eloret Corporation at National Aeronautic and Space Administration, Ames Research Center, Moffett Field, CA 94035; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143; and Systemix Institute, Cupertino, CA 95014"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,2,28]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(02)02410-8"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M200037-MCP200"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00450-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00412-X"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.02.110186.002505"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200106141"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1101312"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.132275199"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.229202"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.362402"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(86)34096-5"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5492.809"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103388"},{"key":"e_1_3_2_15_2","unstructured":"Stolc V. 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