{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:19:05Z","timestamp":1792714745763,"version":"4.5.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,4,26]]},"abstract":"<jats:p>\n                    We have created a stable transgenic\n                    <jats:italic>rag2-EGFP-mMyc<\/jats:italic>\n                    zebrafish line that develops GFP-labeled T cell acute lymphoblastic leukemia (T-ALL), allowing visualization of the onset and spread of this disease. Here, we show that leukemias from this transgenic line are highly penetrant and render animals moribund by 80.7 \u00b1 17.6 days of life (\u00b11 SD, range = 50-158 days). These T cell leukemias are clonally aneuploid, can be transplanted into irradiated recipient fish, and express the zebrafish orthologues of the human T-ALL oncogenes\n                    <jats:italic>tal1<\/jats:italic>\n                    \/\n                    <jats:italic>scl<\/jats:italic>\n                    and\n                    <jats:italic>lmo2<\/jats:italic>\n                    , thus providing an animal model for the most prevalent molecular subgroup of human T-ALL. Because T-ALL develops very rapidly in\n                    <jats:italic>rag2-EGFP-mMyc<\/jats:italic>\n                    transgenic fish (in which \u201cmMyc\u201d represents mouse c-Myc), this line can only be maintained by\n                    <jats:italic>in vitro<\/jats:italic>\n                    fertilization. Thus, we have created a conditional transgene in which the\n                    <jats:italic>EGFP-mMyc<\/jats:italic>\n                    oncogene is preceded by a loxed\n                    <jats:italic>dsRED2<\/jats:italic>\n                    gene and have generated stable\n                    <jats:italic>rag2-loxP-dsRED2-loxP-EGFP-mMyc<\/jats:italic>\n                    transgenic zebrafish lines, which have red fluorescent thymocytes and do not develop leukemia. Transgenic progeny from one of these lines can be induced to develop T-ALL by injecting\n                    <jats:italic>Cre<\/jats:italic>\n                    RNA into one-cell-stage embryos, demonstrating the utility of the Cre\/lox system in the zebrafish and providing an essential step in preparing this model for chemical and genetic screens designed to identify modifiers of Myc-induced T-ALL.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0408708102","type":"journal-article","created":{"date-parts":[[2005,4,13]],"date-time":"2005-04-13T01:05:22Z","timestamp":1113354322000},"page":"6068-6073","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":243,"title":["Cre\/lox-regulated transgenic zebrafish model with conditional myc-induced T cell acute lymphoblastic leukemia"],"prefix":"10.1073","volume":"102","author":[{"given":"David M.","family":"Langenau","sequence":"first","affiliation":[{"name":"Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115; Department of Hematology\/Oncology, Children's Hospital, Boston, MA 02115; and Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115; Department of Hematology\/Oncology, Children's Hospital, Boston, MA 02115; and Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephane","family":"Berghmans","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115; Department of Hematology\/Oncology, Children's Hospital, Boston, MA 02115; and Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Kanki","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115; Department of Hematology\/Oncology, Children's Hospital, Boston, MA 02115; and Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffery L.","family":"Kutok","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115; Department of Hematology\/Oncology, Children's Hospital, Boston, MA 02115; and Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Thomas","family":"Look","sequence":"additional","affiliation":[{"name":"Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115; Department of Hematology\/Oncology, Children's Hospital, Boston, MA 02115; and Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,4,12]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(02)00018-1"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5340.1059"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(00)90018-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402249"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.3497"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402892"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2264(2000)9999:9999<::AID-GCC1050>3.0.CO;2-Y"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-07-2577"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(03)00195-1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(02)00032-6"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15542-6"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2001-11-0093"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1102160"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.9.2321.bloodjournal8592321"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080280"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.24.12965"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00482-1"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt963"},{"key":"e_1_3_2_19_2","first-page":"37","volume":"123","year":"1996","unstructured":"Driever, W., Solnica-Krezel, L., Schier, A. 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