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NPC2 has an Ig-like fold stabilized by three disulfide bonds. The structure of the bovine protein reveals a loosely packed region penetrating from the surface into the hydrophobic core that forms adjacent small cavities with a total volume of \u2248160 \u212b\n                    <jats:sup>3<\/jats:sup>\n                    . We propose that this region represents the incipient cholesterol-binding site that dilates to accommodate an \u2248740-\u212b\n                    <jats:sup>3<\/jats:sup>\n                    cholesterol molecule.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0437840100","type":"journal-article","created":{"date-parts":[[2003,3,4]],"date-time":"2003-03-04T13:36:00Z","timestamp":1046784960000},"page":"2512-2517","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":288,"title":["Structure of a cholesterol-binding protein deficient in Niemann\u2013Pick type C2 disease"],"prefix":"10.1073","volume":"100","author":[{"given":"Natalia","family":"Friedland","sequence":"first","affiliation":[{"name":"Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, Departments of Pharmacology and Biochemistry, University of Medicine and Dentistry of New Jersey\u2013Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng-Ling","family":"Liou","sequence":"additional","affiliation":[{"name":"Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, Departments of Pharmacology and Biochemistry, University of Medicine and Dentistry of New Jersey\u2013Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Lobel","sequence":"additional","affiliation":[{"name":"Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, Departments of Pharmacology and Biochemistry, University of Medicine and Dentistry of New Jersey\u2013Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann M.","family":"Stock","sequence":"additional","affiliation":[{"name":"Center for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute, Departments of Pharmacology and Biochemistry, University of Medicine and Dentistry of New Jersey\u2013Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2003,2,18]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"2863","volume-title":"The Metabolic and Molecular Bases of Inherited Disease","author":"Goldstein J L","year":"2001","unstructured":"J L Goldstein, H H Hobbs, M S Brown The Metabolic and Molecular Bases of Inherited Disease, eds C R Scriver, A L Beaudet, W S Sly, D Valle (McGraw\u2013Hill, New York) III, 2863\u20132913 (2001)."},{"key":"e_1_3_4_2_2","first-page":"3611","volume-title":"The Metabolic and Molecular Bases of Inherited Disease","author":"Patterson M C","year":"2001","unstructured":"M C Patterson, M T Vanier, K Suzuki, J A Morris, E Carstea, E B Neufeld, J E Blanchette-Mackie, P G Pentchev The Metabolic and Molecular Bases of Inherited Disease, eds C R Scriver, A L Beaudet, W S Sly, D Valle (McGraw\u2013Hill, New York) III, 3611\u20133633 (2001)."},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1126\/science.277.5323.228","volume":"277","author":"Carstea E D","year":"1997","unstructured":"E D Carstea, J A Morris, K G Coleman, S K Loftus, D Zhang, C Cummings, J Gu, M A Rosenfeld, W J Pavan, D B Krizman, et al. 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