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Na\u00efve B cells, characterized by the expression of cell cycle-inhibitory and antiapoptotic genes, become centroblasts by inducing an atypical proliferation program lacking c-Myc expression, switching to a proapoptotic program, and down-regulating cytokine, chemokine, and adhesion receptors. The transition from GC to memory cells is characterized by a return to a phenotype similar to that of na\u00efve cells except for an apoptotic program primed for both death and survival and for changes in the expression of cell surface receptors including IL-2 receptor \u03b2. These results provide insights into the dynamics of the GC reaction and represent the basis for the analysis of B cell malignancies.<\/jats:p>","DOI":"10.1073\/pnas.0437996100","type":"journal-article","created":{"date-parts":[[2003,3,4]],"date-time":"2003-03-04T13:36:00Z","timestamp":1046784960000},"page":"2639-2644","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":366,"title":["Transcriptional analysis of the B cell germinal center reaction"],"prefix":"10.1073","volume":"100","author":[{"given":"Ulf","family":"Klein","sequence":"first","affiliation":[{"name":"Institute for Cancer Genetics, and Departments of Pathology and Genetics and Development, Columbia University, New York, NY 10032; IBM T. J. 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