{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:39:51Z","timestamp":1781109591224,"version":"3.54.1"},"reference-count":46,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,4,26]]},"abstract":"<jats:p>\n                    The restriction of influenza A virus replication to mouse respiratory epithelium means that this host response is anatomically compartmentalized, on the one hand, to sites of T cell stimulation and proliferation in the secondary lymphoid tissue and, on the other hand, to the site of effector T cell function and pathology in the pneumonic lung. Thus, it is hardly surprising that virus-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells recovered by bronchoalveolar lavage (BAL) from the infected respiratory tract seem more \u201cactivated\u201d in terms of both cytolytic activity and cytokine production than those cells isolated from the spleen. The present analysis uses Affymetrix microarray technology to compare profiles of gene expression in these two lineage-related, yet anatomically separate, lymphocyte populations. Ninety differentially expressed genes were identified for influenza-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    D\n                    <jats:sup>b<\/jats:sup>\n                    NP\n                    <jats:sub>366<\/jats:sub>\n                    <jats:sup>+<\/jats:sup>\n                    T cells obtained directly\n                    <jats:italic>ex vivo<\/jats:italic>\n                    by BAL or spleen disruption, with nine genes being further analyzed by quantitative, real-time PCR at the population level. Integrin \u03b1E, for example, was shown by Affymetrix and real-time mRNA analyses and then by single-cell PCR and protein staining to be present at a much higher prevalence on the BAL CD8\n                    <jats:sup>+<\/jats:sup>\n                    D\n                    <jats:sup>b<\/jats:sup>\n                    NP\n                    <jats:sub>366<\/jats:sub>\n                    <jats:sup>+<\/jats:sup>\n                    set. The unpredicted finding, however, was that mRNA expression for 75% of the 90 genes was lower in T cells from the BAL than from the spleen. Apparently, the localization of virus-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells to the site of virus-induced pathology is associated with a narrowing, or \u201cfocusing,\u201d of gene expression that favors enhanced effector function in the damaged, \u201chigh-antigen load\u201d environment of the pneumonic lung.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0501960102","type":"journal-article","created":{"date-parts":[[2005,4,14]],"date-time":"2005-04-14T20:24:21Z","timestamp":1113510261000},"page":"6074-6079","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":21,"title":["Effector CD8\n                    <sup>+<\/sup>\n                    T cells recovered from an influenza pneumonia differentiate to a state of focused gene expression"],"prefix":"10.1073","volume":"102","author":[{"given":"Dana R.","family":"Marshall","sequence":"first","affiliation":[{"name":"Departments of Immunology, Experimental Hematology, and Biostatistics and Hartwell Center for Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105; 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and Department of Microbiology and Immunology, University of Melbourne, Parkville VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzette","family":"Wingo","sequence":"additional","affiliation":[{"name":"Departments of Immunology, Experimental Hematology, and Biostatistics and Hartwell Center for Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105; and Department of Microbiology and Immunology, University of Melbourne, Parkville VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Departments of Immunology, Experimental Hematology, and Biostatistics and Hartwell Center for Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105; and Department of Microbiology and Immunology, University of Melbourne, Parkville VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Doherty","sequence":"additional","affiliation":[{"name":"Departments of Immunology, Experimental Hematology, and Biostatistics and Hartwell Center for Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105; 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