{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:57:16Z","timestamp":1791356236914,"version":"4.3.1"},"reference-count":29,"publisher":"National Academy of Sciences","issue":"20","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,5,17]]},"abstract":"<jats:p>\n                    Functional and phenotypic characterization of virus-specific CD8 T cells against cytomegalovirus, Epstein-Barr virus, influenza (flu), and HIV-1 were performed on the basis of the ability of CD8 T cells to secrete IFN-\u03b3 and IL-2, to proliferate, and to express CD45RA and CCR7. Two functional distinct populations of CD8 T cells were identified: (\n                    <jats:italic>i<\/jats:italic>\n                    ) dual IFN-\u03b3\/IL-2-secreting cells and (\n                    <jats:italic>ii<\/jats:italic>\n                    ) single IFN-\u03b3-secreting cells. Virus-specific IFN-\u03b3\/IL-2-secreting CD8 T cells were CD45RA\n                    <jats:sup>-<\/jats:sup>\n                    CCR7\n                    <jats:sup>-<\/jats:sup>\n                    , whereas single IFN-\u03b3 CD8 T cells were either CD45RA\n                    <jats:sup>-<\/jats:sup>\n                    CCR7\n                    <jats:sup>-<\/jats:sup>\n                    or CD45RA\n                    <jats:sup>+<\/jats:sup>\n                    CCR7\n                    <jats:sup>-<\/jats:sup>\n                    . The proportion of virus-specific IFN-\u03b3\/IL-2-secreting CD8 T cells correlated with that of proliferating CD8 T cells, and the loss of HIV-1-specific IL-2-secreting CD8 T cells was associated with that of HIV-1-specific CD8 T cell proliferation. Substantial proliferation of virus-specific CD8 T cells (including HIV-1-specific CD8 T cells) was also observed in CD4 T cell-depleted populations or after stimulation with MHC class I tetramer-peptide complexes. IL-2 was the factor responsible for the CD4-independent CD8 T cell proliferation. These results indicate that IFN-\u03b3\/IL-2-secreting CD8 T cells may promote antigen-specific proliferation of CD8 T cells even in the absence of helper CD4 T cells.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0502393102","type":"journal-article","created":{"date-parts":[[2005,5,4]],"date-time":"2005-05-04T20:25:09Z","timestamp":1115238309000},"page":"7239-7244","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":247,"title":["HIV-1-specific IFN-\u03b3\/IL-2-secreting CD8 T cells support CD4-independent proliferation of HIV-1-specific CD8 T cells"],"prefix":"10.1073","volume":"102","author":[{"given":"Simone C.","family":"Zimmerli","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Immunology and Allergy, Laboratory of AIDS Immunopathogenesis, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Harari","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Immunology and Allergy, Laboratory of AIDS Immunopathogenesis, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Cellerai","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Immunology and Allergy, Laboratory of AIDS Immunopathogenesis, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Vallelian","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Immunology and Allergy, Laboratory of AIDS Immunopathogenesis, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Alexandre","family":"Bart","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Immunology and Allergy, Laboratory of AIDS Immunopathogenesis, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Pantaleo","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Immunology and Allergy, Laboratory of AIDS Immunopathogenesis, Centre Hospitalier Universitaire Vaudois, University of Lausanne, 1011 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,5,4]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.21.120601.141114"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5258.54"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.561"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri778"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01139-X"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni969"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.100101.151926"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/44385"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.7.4204"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.6.3963"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.1.181"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni845"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041270"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-04-1203"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031598"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.20.10900-10909.2003"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.11.7569-7579.1996"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.405"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.6.859"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.23.9514"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.4049\/jimmunol.147.5.1512","volume":"147","year":"1991","unstructured":"Johnson, R. P., Trocha, A., Yang, L., Mazzara, G. P., Panicali, D. L., Buchanan, T. M. & Walker, B. D. (1991) J. Immunol. 147, 1512-1521.1715361","journal-title":"J. Immunol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/2.8.707"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1993.9.1217"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(01)00535-X"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/35065118"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200212)32:12<3756::AID-IMMU3756>3.0.CO;2-E"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni889"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.8.4911-4927.2003"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1105"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0502393102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T00:43:46Z","timestamp":1683074626000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0502393102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,5,4]]},"references-count":29,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2005,5,17]]}},"alternative-id":["10.1073\/pnas.0502393102"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0502393102","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,5,4]]},"assertion":[{"value":"2004-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-05-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}