{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:12:44Z","timestamp":1791306764620,"version":"4.3.1"},"reference-count":49,"publisher":"National Academy of Sciences","issue":"32","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,8,9]]},"abstract":"<jats:p>\n                    Sequestration of malaria-parasite-infected erythrocytes in the microvasculature of organs is thought to be a significant cause of pathology. Cerebral malaria (CM) is a major complication of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    infections, and PfEMP1-mediated sequestration of infected red blood cells has been considered to be the major feature leading to CM-related pathology. We report a system for the real-time\n                    <jats:italic>in vivo<\/jats:italic>\n                    imaging of sequestration using transgenic luciferase-expressing parasites of the rodent malaria parasite\n                    <jats:italic>Plasmodium berghei.<\/jats:italic>\n                    These studies revealed that: (\n                    <jats:italic>i<\/jats:italic>\n                    ) as expected, lung tissue is a major site, but, unexpectedly, adipose tissue contributes significantly to sequestration, and (\n                    <jats:italic>ii<\/jats:italic>\n                    ) the class II scavenger-receptor CD36 to which PfEMP1 can bind is also the major receptor for\n                    <jats:italic>P. berghei<\/jats:italic>\n                    sequestration, indicating a role for alternative parasite ligands, because orthologues of PfEMP1 are absent from rodent malaria parasites, and, importantly, (\n                    <jats:italic>iii<\/jats:italic>\n                    ) cerebral complications still develop in the absence of CD36-mediated sequestration, dissociating parasite sequestration from CM-associated pathology. Real-time\n                    <jats:italic>in vivo<\/jats:italic>\n                    imaging of parasitic processes may be used to evaluate the molecular basis of pathology and develop strategies to prevent pathology.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0503386102","type":"journal-article","created":{"date-parts":[[2005,7,28]],"date-time":"2005-07-28T20:38:31Z","timestamp":1122583111000},"page":"11468-11473","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":280,"title":["Murine malaria parasite sequestration: CD36 is the major receptor, but cerebral pathology is unlinked to sequestration"],"prefix":"10.1073","volume":"102","author":[{"given":"Blandine","family":"Franke-Fayard","sequence":"first","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris J.","family":"Janse","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarida","family":"Cunha-Rodrigues","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jai","family":"Ramesar","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"B\u00fcscher","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivo","family":"Que","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemens","family":"L\u00f6wik","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Voshol","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion A. M.","family":"den Boer","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sjoerd G.","family":"van Duinen","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Febbraio","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria M.","family":"Mota","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew P.","family":"Waters","sequence":"additional","affiliation":[{"name":"Departments of Parasitology, Endocrinology and Metabolic Diseases, and Pathology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands; Instituto Gulbenkian de Ci\u00eancia, P-2781-901 Oeiras, Portugal; Department of Parasitology, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium; and Department of Cell Biology, Lerner Research Institute, Cleveland, OH 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,7,28]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.6.C1231"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(03)00162-X"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/340213"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2004.11.008"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2004.09.006"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(02)01541-1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(03)00229-1"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.7861\/clinmedicine.1-6-495"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2003.08.006"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2467377"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/21900"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.151028698"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.54.1.157"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/5013"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.49.12.2222"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.27.19055"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M300143-JLR200"},{"key":"e_1_3_2_18_2","first-page":"25","volume":"30","year":"2001","unstructured":"Chatterjee, S., Ngonseu, E., Van Overmeir, C., Correwyn, A., Druilhe, P. & Wery, M. 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