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This induced fit dramatically alters the electrostatics of the active site, creating a binding pocket for aspartate. Upon aspartate binding, a further change in electrostatics causes a second induced fit, the domain closure. This domain closure acts as a clamp that both facilitates catalysis by approximation and also initiates the global conformational change that manifests homotropic cooperativity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0503742102","type":"journal-article","created":{"date-parts":[[2005,6,10]],"date-time":"2005-06-10T20:25:46Z","timestamp":1118435146000},"page":"8881-8886","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":45,"title":["Structural basis for ordered substrate binding and cooperativity in aspartate transcarbamoylase"],"prefix":"10.1073","volume":"102","author":[{"given":"Jie","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Chemistry, Boston College, Merkert Chemistry Center, Chestnut Hill, MA 02467"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly A.","family":"Stieglitz","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Boston College, Merkert Chemistry Center, Chestnut Hill, MA 02467"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"Cardia","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Boston College, Merkert Chemistry Center, Chestnut Hill, MA 02467"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan R.","family":"Kantrowitz","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Boston College, Merkert Chemistry Center, Chestnut Hill, MA 02467"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,6,10]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00188a001"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.04.004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.6.1643"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.2.361"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)32593-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)42149-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(74)90454-8"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90365-8"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/87582"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)91759-X"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(87)90498-X"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889897001866"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00786a027"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)44718-2"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)38630-1"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00089a034"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_18_2","first-page":"680","volume":"121","year":"1964","unstructured":"Davis, B. 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