{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:57:21Z","timestamp":1791478641422,"version":"4.3.4"},"reference-count":49,"publisher":"National Academy of Sciences","issue":"28","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,7,12]]},"abstract":"<jats:p>\n                    The satellite sequences (AGGGAG)\n                    <jats:italic>\n                      <jats:sub>n<\/jats:sub>\n                    <\/jats:italic>\n                    and Ty\n                    <jats:italic>3<\/jats:italic>\n                    \/\n                    <jats:italic>gypsy<\/jats:italic>\n                    -like retrotransposons are known to localize at the barley centromeres. Using a gametocidal system, which induces chromosomal mutations in barley chromosomes added to common wheat, we obtained an isochromosome for the short arm of barley chromosome 7H (7HS) that lacked the barley-specific satellite sequence (AGGGAG)\n                    <jats:italic>\n                      <jats:sub>n<\/jats:sub>\n                    <\/jats:italic>\n                    . Two telocentric derivatives of the isochromosome arose in the progeny: 7HS* with and 7HS** without the pericentromeric C-band. FISH analysis demonstrated that both telosomes lacked not only the barley-specific centromeric (AGGGAG)\n                    <jats:italic>\n                      <jats:sub>n<\/jats:sub>\n                    <\/jats:italic>\n                    repeats and retroelements but also any of the known wheat centromeric tandem repeats, including the 192-bp, 250-bp, and TaiI sequences. Although they lacked these centromeric repeats, 7HS* and 7HS** both showed normal mitotic and meiotic transmission. Translocation of barley centromeric repeats to a wheat chromosome 4A did not generate a dicentric chromosome. Indirect immunostaining revealed that all tested centromere-specific proteins (rice CENH3, maize CENP-C, and putative barley homologues of the yeast kinetochore proteins CBF5 and SKP1) and histone H3 phosphorylated at serines 10 and 28 localized at the centromeric region of 7HS*. We conclude that the barley centromeric repeats are neither sufficient nor obligatory to assemble kinetochores, and we discuss the possible formation of a novel centromere in a barley chromosome.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0504235102","type":"journal-article","created":{"date-parts":[[2005,7,5]],"date-time":"2005-07-05T23:23:39Z","timestamp":1120605819000},"page":"9842-9847","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":179,"title":["Stable barley chromosomes without centromeric repeats"],"prefix":"10.1073","volume":"102","author":[{"given":"S.","family":"Nasuda","sequence":"first","affiliation":[{"name":"Laboratory of Plant Genetics, Graduate School of Agriculture and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; and Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Hudakova","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Genetics, Graduate School of Agriculture and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; and Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Schubert","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Genetics, Graduate School of Agriculture and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; and Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Houben","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Genetics, Graduate School of Agriculture and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; and Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. R.","family":"Endo","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Genetics, Graduate School of Agriculture and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; and Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,7,5]]},"reference":[{"key":"e_1_3_2_1_2","unstructured":"Kalitsis P. & Choo K. H. A. (1997) in The Centromere ed. Choo K. H. A. (Oxford Univ. Press Oxford) pp. 97\u2013142."},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2003.09.007"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02524643"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.14210"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00341.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.22.13073"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.14.8135"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/150.4.1615"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/156.1.313"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pce026"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.162301"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.24.5029"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1139\/g01-092"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.003079"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/163.2.759"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.006106"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.018937"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/342730"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/158.4.1615"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00412-002-0224-2"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.7.4490"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010373"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.11.7.1227"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a110529"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1266\/jjg.65.135"},{"key":"e_1_3_2_26_2","unstructured":"Endo T. R. Shi F. & Tsvetkov K. S. (1998) in Proceedings of the 9th International Wheat Genetics Symposium Saskatoon SK Canada ed. Slinkard A. E. (Univ. Extension Press Univ. Saskatchewan Saskatoon Canada) pp. 40\u201343."},{"key":"e_1_3_2_27_2","unstructured":"Endo T. R. (2003) in Proceedings of the 10th International Wheat Genetics Symposium Peastum Italy ed. Pogna N. E. (Istituto Sperimentale per la Cerealicoltura Rome) pp. 69\u201372."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1266\/ggs.74.49"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004120000085"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/hdy.1981.24"},{"key":"e_1_3_2_31_2","unstructured":"Islam A. K. M. R. (1983) in Proceedings of the 6th International Wheat Genetics Symposium Kyoto Japan ed. Sakamoto S. (Plant Germplasm Institute Kyoto Univ. Kyoto) pp. 233\u2013238."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1139\/g91-128"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1139\/g04-034"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/164.2.665"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1139\/g02-059"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00210075"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1998.00125.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016845200960"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.36.25543"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1999.00496.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1139\/g93-053"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009876621187"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004120000109"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1289"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1159\/000074175"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004120000087"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a109623"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.4.831"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(03)00019-4"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0504235102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T14:18:51Z","timestamp":1649773131000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0504235102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,7,5]]},"references-count":49,"journal-issue":{"issue":"28","published-print":{"date-parts":[[2005,7,12]]}},"alternative-id":["10.1073\/pnas.0504235102"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0504235102","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,7,5]]},"assertion":[{"value":"2004-08-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-07-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}