{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:06:11Z","timestamp":1791295571539,"version":"4.1.0"},"reference-count":37,"publisher":"National Academy of Sciences","issue":"39","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,9,27]]},"abstract":"<jats:p>\n                    Bacteria of\n                    <jats:italic>Shigella<\/jats:italic>\n                    spp. are responsible for shigellosis in humans. They use a type III secretion system to inject effector proteins into host cells and induce their entry into epithelial cells or trigger apoptosis in macrophages. We present evidence that the effector OspG is a protein kinase that binds various ubiquitinylated ubiquitin-conjugating enzymes, including UbcH5, which belongs to the stem cell factor SCF\n                    <jats:sup>\u03b2-TrCP<\/jats:sup>\n                    complex promoting ubiquitination of phosphorylated inhibitor of NF-\u03baB type \u03b1 (phospho-I\u03baB\u03b1). Transfection experiments indicated that OspG can prevent phospho-I\u03baB\u03b1 degradation and NF-\u03baB activation induced by TNF-\u03b1 stimulation. Infection of epithelial cells by the\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>flexneri<\/jats:italic>\n                    wild-type strain, but not an\n                    <jats:italic>ospG<\/jats:italic>\n                    mutant, led to accumulation of phospho-I\u03baB\u03b1, consistent with OspG inhibiting SCF\n                    <jats:sup>\u03b2-TrCP<\/jats:sup>\n                    activity. Upon infection of ileal loops in rabbits, the\n                    <jats:italic>ospG<\/jats:italic>\n                    mutant induced a stronger inflammatory response than the wild-type strain. This finding indicates that OspG negatively controls the host innate response induced by\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>flexneri<\/jats:italic>\n                    upon invasion of the epithelium.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0504466102","type":"journal-article","created":{"date-parts":[[2005,9,14]],"date-time":"2005-09-14T20:25:13Z","timestamp":1126729513000},"page":"14046-14051","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":306,"title":["The\n                    <i>Shigella flexneri<\/i>\n                    effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes"],"prefix":"10.1073","volume":"102","author":[{"given":"Dong Wook","family":"Kim","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 Mixte de Recherche 389, Institut Pasteur, 28 Rue du Dr. Roux, 75015 Paris, France; and Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerlinde","family":"Lenzen","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 Mixte de Recherche 389, Institut Pasteur, 28 Rue du Dr. Roux, 75015 Paris, France; and Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Laure","family":"Page","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 Mixte de Recherche 389, Institut Pasteur, 28 Rue du Dr. Roux, 75015 Paris, France; and Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Legrain","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 Mixte de Recherche 389, Institut Pasteur, 28 Rue du Dr. Roux, 75015 Paris, France; and Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe J.","family":"Sansonetti","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 Mixte de Recherche 389, Institut Pasteur, 28 Rue du Dr. Roux, 75015 Paris, France; and Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Parsot","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Unit\u00e9 Mixte de Recherche 389, Institut Pasteur, 28 Rue du Dr. Roux, 75015 Paris, France; and Hybrigenics, 3-5 Impasse Reille, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,9,14]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1499"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri910"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.621"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.70.1.503"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/416703a"},{"key":"e_1_3_2_6_2","first-page":"651","volume":"77","year":"1999","unstructured":"Kotloff, K. L., Winickoff, J. P., Ivanoff, B., Clemens, J. D., Swerdlow, D. L., Sansonetti, P. J., Adak, G. K. & Levine, M. M. (1999) Bull. W. H. O. 77, 651-666.10516787","journal-title":"Bull. W. H. O."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090124"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.2.379-433.1998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02179.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06863.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.10.2894"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02836.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04645.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27639-0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb00885.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011028200"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.51.30408"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2334104"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.2.903"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.12.8035-8044.1994"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.10.2717"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.4.1307"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.8.7768349"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)00126-H"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.11.3472-3479.2004"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-2985(97)80023-X"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1018"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5484.1560"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2004.00452.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117015"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1084677"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.10.5240"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303749200"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.5.505"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(98)80175-1"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"5063","DOI":"10.4049\/jimmunol.162.9.5063","volume":"162","year":"1999","unstructured":"Kojima, H., Aizawa, Y., Yanai, Y., Nagaoka, K., Takeuchi, M., Ohta, T., Ikegami, H., Ikeda, M. & Kurimoto, M. (1999) J. Immunol. 162, 5063-5069.10227974","journal-title":"J. Immunol."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/32588"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0504466102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T09:55:53Z","timestamp":1683194153000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0504466102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9,14]]},"references-count":37,"journal-issue":{"issue":"39","published-print":{"date-parts":[[2005,9,27]]}},"alternative-id":["10.1073\/pnas.0504466102"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0504466102","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1028471.342301","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1028471.577047","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1028471.342165","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1028471.341119","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9,14]]},"assertion":[{"value":"2005-05-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-07-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-09-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}