{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T19:33:53Z","timestamp":1793043233475,"version":"4.5.0"},"reference-count":24,"publisher":"National Academy of Sciences","issue":"44","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:p>\n                    Herpes simplex virus (HSV) persists in its human host and evades the immune response by undergoing a latent infection in sensory neurons, from which it can reactivate periodically. HSV expresses &gt;80 gene products during productive (\u201clytic\u201d) infection, but only the latency-associated transcript (\n                    <jats:italic>LAT<\/jats:italic>\n                    ) gene is expressed at abundant levels during latent infection. The\n                    <jats:italic>LAT<\/jats:italic>\n                    gene has been shown to repress lytic-gene expression in sensory neurons. In this study, we use chromatin immunoprecipitation to show that HSV lytic-gene promoters become complexed with modified histones associated with heterochromatin during the course of establishment of latent infection. Experiments comparing LAT-negative and LAT-positive viruses show that a function encoded by the\n                    <jats:italic>LAT<\/jats:italic>\n                    gene increases the amount of dimethyl lysine 9 form of histone H3 or heterochromatin and reduces the amount of dimethyl lysine 4 form of histone H3, a part of active chromatin, on viral lytic-gene promoters. Thus, HSV, and in particular the HSV\n                    <jats:italic>LAT<\/jats:italic>\n                    gene, may manipulate the cellular histone modification machinery to repress its lytic-gene expression and contribute to the persistence of its genome in a quiescent form in sensory neurons.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0505850102","type":"journal-article","created":{"date-parts":[[2005,10,26]],"date-time":"2005-10-26T04:43:54Z","timestamp":1130301834000},"page":"16055-16059","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":246,"title":["Herpesviral latency-associated transcript gene promotes assembly of heterochromatin on viral lytic-gene promoters in latent infection"],"prefix":"10.1073","volume":"102","author":[{"given":"Qing-Yin","family":"Wang","sequence":"first","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics and Biological Chemistry and Molecular Pharmacology and Program in Virology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics and Biological Chemistry and Molecular Pharmacology and Program in Virology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen E.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics and Biological Chemistry and Molecular Pharmacology and Program in Virology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C.","family":"Colgrove","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics and Biological Chemistry and Molecular Pharmacology and Program in Virology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald M.","family":"Coen","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics and Biological Chemistry and Molecular Pharmacology and Program in Virology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Knipe","sequence":"additional","affiliation":[{"name":"Departments of Microbiology and Molecular Genetics and Biological Chemistry and Molecular Pharmacology and Program in Virology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,10,24]]},"reference":[{"key":"e_1_3_2_1_2","unstructured":"Roizman B. & Knipe D. 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