{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T12:00:37Z","timestamp":1789819237278,"version":"4.0.1"},"reference-count":31,"publisher":"National Academy of Sciences","issue":"40","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,10,4]]},"abstract":"<jats:p>\n                    PKC-\u03b8 is central to T-helper (Th) 2 cell differentiation and effector function; however, its importance for antiviral effector, and in particular memory CD8+ T cell responses, remains unclear. We have investigated the role of PKC-\u03b8 during\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    responses against influenza virus, lymphocytic choriomeningitis virus, vaccinia virus, and replication-deficient virus-like particles. In the absence of PKC-\u03b8, antiviral CD8+ T cells presented an unresponsive phenotype\n                    <jats:italic>in vitro<\/jats:italic>\n                    , which could be restored with exogenous IL-2 or by Toll-like receptor ligand-activated dendritic cells. In striking contrast, PKC-\u03b8 appeared to be superfluous for\n                    <jats:italic>in vivo<\/jats:italic>\n                    antiviral responses irrespective of whether the virus infected systemically, was localized to the lung, or did not replicate. In addition, CD8+ CCR7-effector memory responses were normal in PKC-\u03b8-deficient mice, both in lymphoid and peripheral tissues. Our data show that increased activation signals delivered\n                    <jats:italic>in vivo<\/jats:italic>\n                    by highly activated dendritic cells, as present during viral infections, overcome the requirement for PKC-\u03b8 during CD8+ T cell antiviral responses.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0506250102","type":"journal-article","created":{"date-parts":[[2005,9,26]],"date-time":"2005-09-26T20:35:00Z","timestamp":1127766900000},"page":"14374-14379","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":48,"title":["Innate signals compensate for the absence of PKC-\u03b8 during\n                    <i>in vivo<\/i>\n                    CD8+ T cell effector and memory responses"],"prefix":"10.1073","volume":"102","author":[{"given":"Benjamin J.","family":"Marsland","sequence":"first","affiliation":[{"name":"Molecular Biomedicine, Swiss Federal Institute of Technology, Wagistrasse 27, CH-8952 Z\u00fcrich-Schlieren, Switzerland; Universit\u00e4t Schleswig-Holstein, Campus Kiel, Klinik f\u00fcr Nephrologie, Forschungslabor, Fleckenstrasse 4, D-24105 Kiel, Germany; and Cytos Biotechnology AG, Wagistrasse 25, CH-8952 Z\u00fcrich-Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Nembrini","sequence":"additional","affiliation":[{"name":"Molecular Biomedicine, Swiss Federal Institute of Technology, Wagistrasse 27, CH-8952 Z\u00fcrich-Schlieren, Switzerland; Universit\u00e4t Schleswig-Holstein, Campus Kiel, Klinik f\u00fcr Nephrologie, Forschungslabor, Fleckenstrasse 4, D-24105 Kiel, Germany; and Cytos Biotechnology AG, Wagistrasse 25, CH-8952 Z\u00fcrich-Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Molecular Biomedicine, Swiss Federal Institute of Technology, Wagistrasse 27, CH-8952 Z\u00fcrich-Schlieren, Switzerland; Universit\u00e4t Schleswig-Holstein, Campus Kiel, Klinik f\u00fcr Nephrologie, Forschungslabor, Fleckenstrasse 4, D-24105 Kiel, Germany; and Cytos Biotechnology AG, Wagistrasse 25, CH-8952 Z\u00fcrich-Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Abel","sequence":"additional","affiliation":[{"name":"Molecular Biomedicine, Swiss Federal Institute of Technology, Wagistrasse 27, CH-8952 Z\u00fcrich-Schlieren, Switzerland; Universit\u00e4t Schleswig-Holstein, Campus Kiel, Klinik f\u00fcr Nephrologie, Forschungslabor, Fleckenstrasse 4, D-24105 Kiel, Germany; and Cytos Biotechnology AG, Wagistrasse 25, CH-8952 Z\u00fcrich-Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Krautwald","sequence":"additional","affiliation":[{"name":"Molecular Biomedicine, Swiss Federal Institute of Technology, Wagistrasse 27, CH-8952 Z\u00fcrich-Schlieren, Switzerland; Universit\u00e4t Schleswig-Holstein, Campus Kiel, Klinik f\u00fcr Nephrologie, Forschungslabor, Fleckenstrasse 4, D-24105 Kiel, Germany; and Cytos Biotechnology AG, Wagistrasse 25, CH-8952 Z\u00fcrich-Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin F.","family":"Bachmann","sequence":"additional","affiliation":[{"name":"Molecular Biomedicine, Swiss Federal Institute of Technology, Wagistrasse 27, CH-8952 Z\u00fcrich-Schlieren, Switzerland; Universit\u00e4t Schleswig-Holstein, Campus Kiel, Klinik f\u00fcr Nephrologie, Forschungslabor, Fleckenstrasse 4, D-24105 Kiel, Germany; and Cytos Biotechnology AG, Wagistrasse 25, CH-8952 Z\u00fcrich-Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Kopf","sequence":"additional","affiliation":[{"name":"Molecular Biomedicine, Swiss Federal Institute of Technology, Wagistrasse 27, CH-8952 Z\u00fcrich-Schlieren, Switzerland; Universit\u00e4t Schleswig-Holstein, Campus Kiel, Klinik f\u00fcr Nephrologie, Forschungslabor, Fleckenstrasse 4, D-24105 Kiel, Germany; and Cytos Biotechnology AG, Wagistrasse 25, CH-8952 Z\u00fcrich-Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,9,26]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/25764"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35006090"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020234"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35099560"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400937101"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/35047123"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/35100529"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425730"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20032229"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830240944"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.6.2880"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(99)00034-4"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2004.01859.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.9.3291"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031022"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.12.6296"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.5.2635"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"3980","DOI":"10.4049\/jimmunol.144.10.3980","volume":"144","year":"1990","unstructured":"Allan, W., Tabi, Z., Cleary, A. & Doherty, P. 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