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Among the 116 genes identified, several host pathways previously unrecognized as playing a role in listerial pathogenesis were identified: knockdowns affecting vacuolar trafficking to and from the multivesicular body bypassed the requirement for the essential pore-forming toxin listeriolysin O in mediating escape from phagocytic vacuoles and knockdowns affecting either subunit of serine palmitoyltransferase, a key enzyme in ceramide and sphingolipid biosynthesis, enhanced the toxicity of listeriolysin O expressed in the host cell cytosol, leading to lack of appropriate toxin activity compartmentalization and host cell death. Genome-wide RNA interference screens using\n                    <jats:italic>Drosophila<\/jats:italic>\n                    S2 cells proved to be a powerful approach to dissect host-pathogen interactions.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0506461102","type":"journal-article","created":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T20:43:59Z","timestamp":1126557839000},"page":"13646-13651","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":116,"title":["Use of RNA interference in\n                    <i>Drosophila<\/i>\n                    S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen"],"prefix":"10.1073","volume":"102","author":[{"given":"Luisa W.","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology and the School of Public Health, 508 Barker Hall, University of California, Berkeley, CA 94720-3202; andHoward Hughes Medical Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94107"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie P. 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