{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:01:48Z","timestamp":1792202508927,"version":"4.4.0"},"reference-count":39,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,2,14]]},"abstract":"<jats:p>\n                    Mycobactins are a family of membrane-associated siderophores required for\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    to adapt to its intracellular habitat. These lipophilic siderophores have been recently shown to directly acquire intracellular iron through lipid trafficking. Despite tremendous progress in understanding the assembly-line enzymology of the siderophore biosynthesis, the genes as well as the mechanistic and biochemical principles involved in producing membrane-associated siderophores have not been investigated. Here, we report a biosynthetic locus that incorporates variety of aliphatic chains on the mycobactin skeleton. Cell-free reconstitution studies demonstrate that these acyl chains are directly transferred from a carrier protein on to the \u03b5-amino group of lysine residue by an unidentified Rv1347c gene product. The unsaturation in the lipidic chain is produced by a novel acyl-acyl carrier protein dehydrogenase, which, in contrast to the conventional acyl-CoA dehydrogenases, is involved in the biosynthetic pathway. MbtG protein then performs the final N6-hydroxylation step. Genome-wide analysis revealed homologues of\n                    <jats:italic>N<\/jats:italic>\n                    -acyl transferase and MbtG in other pathogenic bacteria. Because iron plays a key role in the development of infectious diseases, the biosynthetic pathway described here represents an attractive target for developing new antibacterial agents.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0507924103","type":"journal-article","created":{"date-parts":[[2006,2,3]],"date-time":"2006-02-03T20:48:52Z","timestamp":1138999732000},"page":"2069-2074","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":125,"title":["A genetic locus required for iron acquisition in\n                    <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1073","volume":"103","author":[{"given":"R.","family":"Krithika","sequence":"first","affiliation":[{"name":"Chemical Biology Group, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uttara","family":"Marathe","sequence":"additional","affiliation":[{"name":"Chemical Biology Group, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priti","family":"Saxena","sequence":"additional","affiliation":[{"name":"Chemical Biology Group, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd. Zeeshan","family":"Ansari","sequence":"additional","affiliation":[{"name":"Chemical Biology Group, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debasisa","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Chemical Biology Group, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh S.","family":"Gokhale","sequence":"additional","affiliation":[{"name":"Chemical Biology Group, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,2,3]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio706"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.9.3367-3372.2004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(03)00055-X"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1046"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0637444100"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5456.1245"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/br.34.2.99-125.1970"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.66.2.223-249.2002"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.15.4443-4451.1999"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2003.08.012"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.4.1527"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio717"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja026768w"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.3.1252"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(98)90291-5"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03425.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-12-3873"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03384.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02384"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03946.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03948.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.165.2.570-578.1986"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406410101"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/16.4.404"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413904200"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.2.374-382.2004"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.02.009"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5413.482"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(90)90080-S"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1089353"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1515\/BC.1999.006"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.3.589-596.1993"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231275498"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2003.10.021"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2850-2857.2004"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041242"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4443-4455.1999"},{"key":"e_1_3_3_38_2","unstructured":"E. Atherton R. C. Sheppard Solid Phase Peptide Synthesis: A Practical Approach.  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