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These contact points were consistent with the lowest energy LF-PA complex found by using Rosetta protein-protein docking. These results illustrate how biochemical and computational methods can be combined to produce reliable models of large complexes. The model shows that EF and LF bind through a highly electrostatic interface, with their flexible N-terminal region positioned at the entrance of the heptameric PA pore and thus poised to initiate translocation in an N- to C-terminal direction.<\/jats:p>","DOI":"10.1073\/pnas.0508259102","type":"journal-article","created":{"date-parts":[[2005,10,26]],"date-time":"2005-10-26T20:34:03Z","timestamp":1130358843000},"page":"16409-16414","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":47,"title":["A model of anthrax toxin lethal factor bound to protective antigen"],"prefix":"10.1073","volume":"102","author":[{"given":"D. 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