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Here, we report that milk fat globule EGF factor 8 (MFG-E8), a secreted glycoprotein that binds to apoptotic cells by recognizing phosphatidylserine, was expressed by epithelial cells and macrophages in mammary glands and was involved in engulfment of apoptotic cells. A deficiency of\n                    <jats:italic>MFG-E8<\/jats:italic>\n                    caused the accumulation of a large number of milk fat globules (MFGs) in the mammary ducts during involution, indicating that the excess MFGs were cleared by an MFG-E8-dependent mechanism. The\n                    <jats:italic>MFG-E8<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    mice developed mammary duct ectasia with periductal mastitis, and the redevelopment of the mammary gland for their second litter was impaired. These results demonstrate that MFG-E8-mediated phagocytosis of apoptotic epithelial cells and MFGs is important for efficient involution of mammary glands.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0508599102","type":"journal-article","created":{"date-parts":[[2005,11,7]],"date-time":"2005-11-07T20:33:47Z","timestamp":1131395627000},"page":"16886-16891","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":109,"title":["Impaired involution of mammary glands in the absence of milk fat globule EGF factor 8"],"prefix":"10.1073","volume":"102","author":[{"given":"Rikinari","family":"Hanayama","sequence":"first","affiliation":[{"name":"Department of Genetics, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan; Laboratory of Genetics, Integrated Biology Laboratories, Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan; and Solution Oriented Research for Science and Technology, Japan Science and Technology Corporation, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigekazu","family":"Nagata","sequence":"additional","affiliation":[{"name":"Department of Genetics, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan; Laboratory of Genetics, Integrated Biology Laboratories, Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan; and Solution Oriented Research for Science and Technology, Japan Science and Technology Corporation, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2005,11,7]]},"reference":[{"key":"e_1_3_2_1_2","first-page":"181","volume":"122","year":"1996","unstructured":"Lund, L. 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