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DNA repair-deficient XPB and XPD mutant cell lines exhibited an increase in transduction efficiency by both HIV- and Moloney murine leukemia virus-based retroviral vectors. Replicating Moloney murine leukemia virus viral production was greater in XPB or XPD mutant cells but not XPA mutant cells. Quantitative PCR showed an increase in total cDNA molecules, integrated provirus, and 2LTR circles in XPB and XPD mutant cells. In the presence of a reverse transcription inhibitor, the HIV cDNA appeared more stable in mutant XPB or XPD cells. These studies implicate the nuclear DNA repair proteins XPB and XPD in a cellular defense against retroviral infection.<\/jats:p>","DOI":"10.1073\/pnas.0509828103","type":"journal-article","created":{"date-parts":[[2006,3,13]],"date-time":"2006-03-13T20:49:02Z","timestamp":1142282942000},"page":"4622-4627","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":64,"title":["The DNA repair genes XPB and XPD defend cells from retroviral infection"],"prefix":"10.1073","volume":"103","author":[{"given":"Kristine","family":"Yoder","sequence":"first","affiliation":[{"name":"*Department of Molecular Virology, Immunology, and Medical Genetics, and Ohio State University Comprehensive Cancer Center, Ohio State University College of Medicine, Columbus, OH 43210;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Sarasin","sequence":"additional","affiliation":[{"name":"Institut Gustave Roussy, Centre National de la Recherche Scientifique, 94805 Villejuif Cedex, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth","family":"Kraemer","sequence":"additional","affiliation":[{"name":"Basic Research Laboratory, National Cancer Institute, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"McIlhatton","sequence":"additional","affiliation":[{"name":"*Department of Molecular Virology, Immunology, and Medical Genetics, and Ohio State University Comprehensive Cancer Center, Ohio State University College of Medicine, Columbus, OH 43210;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederic","family":"Bushman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Fishel","sequence":"additional","affiliation":[{"name":"*Department of Molecular Virology, Immunology, and Medical Genetics, and Ohio State University Comprehensive Cancer Center, Ohio State University College of Medicine, Columbus, OH 43210;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,3,13]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5414.644"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(03)00455-0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600348"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02458-9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.1.1.409"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1999)14:1<9::AID-HUMU2>3.0.CO;2-6"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.859501"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2003.09.010"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.16.8658"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00182-5"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/S1110724302201023"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.6.1125"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/76095"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5259.263"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.8.3739-3747.2002"},{"key":"e_1_3_3_16_2","first-page":"3837","volume":"54","author":"Gozukara E. 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