{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:05:53Z","timestamp":1791173153571,"version":"4.1.0"},"reference-count":39,"publisher":"National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,1,10]]},"abstract":"<jats:p>\n                    Autoantibody-mediated tissue destruction is among the main features of organ-specific autoimmunity. This report describes \u201can antibody enzyme\u201d (abzyme) contribution to the site-specific degradation of a neural antigen. We detected proteolytic activity toward myelin basic protein (MBP) in the fraction of antibodies purified from the sera of humans with multiple sclerosis (MS) and mice with induced experimental allergic encephalomyelitis. Chromatography and zymography data demonstrated that the proteolytic activity of this preparation was exclusively associated with the antibodies. No activity was found in the IgG fraction of healthy donors. The human and murine abzymes efficiently cleaved MBP but not other protein substrates tested. The sites of MBP cleavage determined by mass spectrometry were localized within immunodominant regions of MBP. The abzymes could also cleave recombinant substrates containing encephalytogenic MBP\n                    <jats:sub>85-101<\/jats:sub>\n                    peptide. An established MS therapeutic Copaxone appeared to be a specific abzyme inhibitor. Thus, the discovered epitope-specific antibody-mediated degradation of MBP suggests a mechanistic explanation of the slow development of neurodegeneration associated with MS.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0509849103","type":"journal-article","created":{"date-parts":[[2005,12,30]],"date-time":"2005-12-30T20:24:39Z","timestamp":1135974279000},"page":"281-286","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":157,"title":["Autoantibodies to myelin basic protein catalyze site-specific degradation of their antigen"],"prefix":"10.1073","volume":"103","author":[{"given":"Natalia A.","family":"Ponomarenko","sequence":"first","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oxana M.","family":"Durova","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan I.","family":"Vorobiev","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Alexey A.","family":"Belogurov","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inna N.","family":"Kurkova","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander G.","family":"Petrenko","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgy B.","family":"Telegin","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey V.","family":"Suchkov","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey L.","family":"Kiselev","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A.","family":"Lagarkova","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim M.","family":"Govorun","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina V.","family":"Serebryakova","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; Vladimirsky Moscow Region Clinical Institute, Ministery of Health, 61\/2, Shchepkina Street, Moscow 129110, Russia; Department of Chemistry, Lomonosov Moscow State University, Vorobievi Gory, Moscow 119899, Russia; Pushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino 142290, Russia;..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9rang\u00e8re","family":"Avalle","sequence":"additional","affiliation":[{"name":"Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10, Miklukho-Maklaya Street, Moscow 117997, Russia; 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