{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:33:43Z","timestamp":1781105623020,"version":"3.54.1"},"reference-count":28,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,3,27]]},"abstract":"<jats:p>\n                    Reovirus genome segment S1 encodes protein \u03c31, which is the\n receptor binding protein, modulates tissue tropism, and specifies the\n nature of the antiviral immune response. It makes up less than 2% of\n reovirus particles and is synthesized in very small amounts in infected\n cells. Any antiviral strategy aimed at reducing specifically the\n expression of this genome segment should, in principle, reduce the\n infectivity of the virus. To test this hypothesis, we have assembled\n two hammer-head motif-containing ribozymes (Rzs) targeted to cleave at\n the conserved B and C domains of the reovirus s1 RNA.\n Protein-independent but Mg\n                    <jats:sup>2+<\/jats:sup>\n                    -dependent\n sequence-specific cleavage of s1 RNA was achieved by both the Rzs\n in trans. Cells that transiently express these Rzs, when challenged\n with reovirus, were protected against the cytopathic effects caused by\n the virus. This protection correlated with the specific intracellular\n reduction of s1 transcripts that was due to their cleavage by the Rzs.\n Rz-treated cells that were challenged with reovirus showed almost\n complete disappearance of protein \u03c31 without significantly altering\n the levels of the other reovirus structural proteins. Thus, Rzs,\n besides acting as antiviral agents, could be exploited as biological\n tools to delineate specific functions of target genes.\n                  <\/jats:p>","DOI":"10.1073\/pnas.051013898","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:44:19Z","timestamp":1027680259000},"page":"4101-4106","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":7,"title":["Ribozymes that cleave reovirus genome segment S1 also protect cells from pathogenesis caused by reovirus infection"],"prefix":"10.1073","volume":"98","author":[{"given":"Shweta","family":"Shahi","sequence":"first","affiliation":[{"name":"Laboratory of Virology, National Institute of Immunology, New\r Delhi-110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganapathy K.","family":"Shanmugasundaram","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, National Institute of Immunology, New\r Delhi-110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhil C.","family":"Banerjea","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, National Institute of Immunology, New\r Delhi-110067, 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