{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:07:59Z","timestamp":1792674479436,"version":"4.5.0"},"reference-count":39,"publisher":"National Academy of Sciences","issue":"3","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,1,16]]},"abstract":"<jats:p>\n                    The navigation of axons toward their targets is a highly dynamic and precisely regulated process during nervous system development. The molecular basis of this navigation process is only partly understood. In\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    , we isolated the RNAi-hypersensitive strain\n                    <jats:italic>nre-1(hd20) lin-15b(hd126)<\/jats:italic>\n                    , which allows us to phenocopy axon guidance defects of known genes by feeding RNAi. We used this mutant strain to systematically screen 4,577 genes on chromosomes I and III for axon guidance phenotypes. We identified 93 genes whose down-regulation led to penetrant ventral cord fasciculation defects or motoneuron commissure outgrowth defects. These genes encode various classes of proteins, ranging from secreted or putative cell surface proteins to transcription factors controlling gene expression. A majority of the genes is evolutionary conserved and previously uncharacterized. In addition, we found axon guidance functions for known genes like\n                    <jats:italic>pry-1<\/jats:italic>\n                    , a component of the Wnt-signaling pathway, and\n                    <jats:italic>ced-1<\/jats:italic>\n                    , a receptor required for the engulfment of neurons undergoing apoptosis during development. Our screen provides insights into molecular pathways operating during the generation of neuronal circuits and provides a basis for a more detailed analysis of gene networks regulating axon navigation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0510527104","type":"journal-article","created":{"date-parts":[[2007,1,9]],"date-time":"2007-01-09T23:37:48Z","timestamp":1168385868000},"page":"834-839","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":123,"title":["Axon guidance genes identified in a large-scale RNAi screen using the RNAi-hypersensitive\n                    <i>Caenorhabditis elegans<\/i>\n                    strain\n                    <i>nre-1(hd20) lin-15b(hd126<\/i>\n                    )"],"prefix":"10.1073","volume":"104","author":[{"given":"Caroline","family":"Schmitz","sequence":"first","affiliation":[{"name":"Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parag","family":"Kinge","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Hutter","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, 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