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An evolutionarily conserved promoter model was generated by comparative genomics in the proximal promoter regions of the slit diaphragm-associated molecule nephrin. Phylogenetic promoter fingerprints of known elements of the slit diaphragm complex identified the nephrin model in the promoter region of zonula occludens-1 (ZO-1). Genome-wide scans using this promoter model effectively predicted a previously unrecognized slit diaphragm molecule, cadherin-5. Nephrin, ZO-1, and cadherin-5 mRNA showed stringent coexpression across a diverse set of human glomerular diseases. Comparative promoter analysis can identify regulatory pathways at work in tissue homeostasis and disease processes.<\/jats:p>","DOI":"10.1073\/pnas.0511257103","type":"journal-article","created":{"date-parts":[[2006,3,31]],"date-time":"2006-03-31T20:25:44Z","timestamp":1143836744000},"page":"5682-5687","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":106,"title":["Comparative promoter analysis allows\n                    <i>de novo<\/i>\n                    identification of specialized cell junction-associated proteins"],"prefix":"10.1073","volume":"103","author":[{"given":"Clemens D.","family":"Cohen","sequence":"first","affiliation":[{"name":"*Medizinische Poliklinik, University of Munich, 80336 Munich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Klingenhoff","sequence":"additional","affiliation":[{"name":"Genomatix GmbH, 80335 Munich, 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