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We report here the crystal structure of a soluble FtsH construct that is functional in caseinolytic and ATPase assays. The molecular architecture of this hexameric molecule consists of two rings where the protease domains possess an all-helical fold and form a flat hexagon that is covered by a toroid built by the AAA domains. The active site of the protease classifies FtsH as an Asp-zincin, contrary to a previous report. The different symmetries of protease and AAA rings suggest a possible translocation mechanism of the target polypeptide chain into the interior of the molecule where the proteolytic sites are located.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0600031103","type":"journal-article","created":{"date-parts":[[2006,2,16]],"date-time":"2006-02-16T20:43:49Z","timestamp":1140122629000},"page":"3066-3071","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":167,"title":["The molecular architecture of the metalloprotease FtsH"],"prefix":"10.1073","volume":"103","author":[{"given":"Christoph","family":"Bieniossek","sequence":"first","affiliation":[{"name":"*Departement f\u00fcr Chemie und Biochemie, Universit\u00e4t Bern, Freiestrasse 3, CH-3012 Bern, Switzerland; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Schalch","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biophysics, Swiss Federal Institute of Technology, Hoenggerberg, HPK Building, CH-8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Bumann","sequence":"additional","affiliation":[{"name":"*Departement f\u00fcr Chemie und Biochemie, Universit\u00e4t Bern, Freiestrasse 3, CH-3012 Bern, Switzerland; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Meister","sequence":"additional","affiliation":[{"name":"*Departement f\u00fcr Chemie und Biochemie, Universit\u00e4t Bern, Freiestrasse 3, CH-3012 Bern, Switzerland; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reto","family":"Meier","sequence":"additional","affiliation":[{"name":"*Departement f\u00fcr Chemie und Biochemie, Universit\u00e4t Bern, Freiestrasse 3, CH-3012 Bern, Switzerland; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Baumann","sequence":"additional","affiliation":[{"name":"*Departement f\u00fcr Chemie und Biochemie, Universit\u00e4t Bern, Freiestrasse 3, CH-3012 Bern, Switzerland; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,2,16]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cell.2004.09.020","volume":"119","author":"Sauer R. 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