{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:03:14Z","timestamp":1792803794608,"version":"4.5.0"},"reference-count":28,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,3,21]]},"abstract":"<jats:p>\n                    Cholera is a waterborne diarrheal disease that continues to plague the developing world. Individuals become infected by consuming water from reservoirs contaminated by virulent strains of the bacterium\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    . Epidemiological and environmental observations of a cholera outbreak in Dhaka, Bangladesh, suggest that lytic bacteriophage specific for\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    may limit the severity of cholera outbreaks by killing bacteria present in the reservoir and in infected individuals. To quantify this idea and generate testable hypotheses, we analyzed a mathematical model that combines the epidemiology of cholera with the population dynamics of the bacteria and phage. Under biologically reasonable conditions, we found that vibriophage can ameliorate cholera outbreaks. If phage predation limits bacterial density before an outbreak, a transient reduction in phage density can disrupt that limitation, and subsequent bacterial growth can initiate a cholera outbreak. The severity of the outbreak depends on the density of phage remaining in the reservoir. If the outbreak is initiated instead by a rise in bacterial density, the introduction of phage can reduce the severity of the outbreak and promote its decline. In both situations, the magnitude of the phage effect depends mainly on vibrio growth and phage mortality rates; the lower the rates, the greater the effect. Our analysis also suggests that either bacteria in the environmental reservoir are hyperinfectious or most victims ingest bacteria amplified in food or drinking water contaminated by environmental water carrying few viable\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    . Our theoretical results make a number of empirically testable predictions.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0600166103","type":"journal-article","created":{"date-parts":[[2006,3,14]],"date-time":"2006-03-14T21:43:40Z","timestamp":1142372620000},"page":"4652-4657","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":187,"title":["Modeling the role of bacteriophage in the control of cholera outbreaks"],"prefix":"10.1073","volume":"103","author":[{"given":"Mark A.","family":"Jensen","sequence":"first","affiliation":[{"name":"*Department of Biology, Emory University, Atlanta, GA 30332;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shah M.","family":"Faruque","sequence":"additional","affiliation":[{"name":"Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research Bangladesh, Dhaka-1212, Bangladesh; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Mekalanos","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce R.","family":"Levin","sequence":"additional","affiliation":[{"name":"*Department of Biology, Emory University, Atlanta, GA 30332;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,3,14]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5270.1910"},{"key":"e_1_3_2_2_2","first-page":"394","volume":"198","author":"Colwell R. 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