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A complementary water-excluding hydrophobic interface and five Ab\u2013Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine.<\/jats:p>","DOI":"10.1073\/pnas.060022997","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:41:48Z","timestamp":1027680108000},"page":"8433-8438","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":95,"title":["Crystal structure of an anti-carbohydrate antibody directed against\n                    <i>Vibrio cholerae<\/i>\n                    O1 in complex with antigen: Molecular basis for serotype specificity"],"prefix":"10.1073","volume":"97","author":[{"given":"S.","family":"Villeneuve","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Biochimie Structurale (Centre National de la Recherche Scientifique, Unit\u00e9 de Recherche Associ\u00e9e 2185), Unit\u00e9 du Chol\u00e9ra et des Vibrions, Unit\u00e9 d'Immunologie Structurale, and Laboratoire d'Ing\u00e9nierie des Anticorps, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France; and Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0815."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Souchon","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biochimie Structurale (Centre National de la Recherche Scientifique, Unit\u00e9 de Recherche Associ\u00e9e 2185), Unit\u00e9 du Chol\u00e9ra et des Vibrions, Unit\u00e9 d'Immunologie Structurale, and Laboratoire d'Ing\u00e9nierie des Anticorps, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France; and Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0815."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.-M.","family":"Riottot","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biochimie Structurale (Centre National de la Recherche Scientifique, Unit\u00e9 de Recherche Associ\u00e9e 2185), Unit\u00e9 du Chol\u00e9ra et des Vibrions, Unit\u00e9 d'Immunologie Structurale, and Laboratoire d'Ing\u00e9nierie des Anticorps, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France; and Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0815."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-C.","family":"Mazi\u00e9","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biochimie Structurale (Centre National de la Recherche Scientifique, Unit\u00e9 de Recherche Associ\u00e9e 2185), Unit\u00e9 du Chol\u00e9ra et des Vibrions, Unit\u00e9 d'Immunologie Structurale, and Laboratoire d'Ing\u00e9nierie des Anticorps, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France; and Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0815."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.-s.","family":"Lei","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biochimie Structurale (Centre National de la Recherche Scientifique, Unit\u00e9 de Recherche Associ\u00e9e 2185), Unit\u00e9 du Chol\u00e9ra et des Vibrions, Unit\u00e9 d'Immunologie Structurale, and Laboratoire d'Ing\u00e9nierie des Anticorps, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France; and Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0815."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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