{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:31:18Z","timestamp":1791250278510,"version":"4.1.0"},"reference-count":46,"publisher":"National Academy of Sciences","issue":"45","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,11,7]]},"abstract":"<jats:p>\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    , the causal agent of Legionnaires' disease, is an intracellular parasite and invades and proliferates within different eukaryotic cells, including human alveolar macrophages. After several 100-fold multiplication within host cells, the pathogens are released for new invasion by induction of apoptosis or necrosis. Here we report that\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    produces a glucosyltransferase, which selectively modifies an \u224850-kDa mammalian protein by using UDP-glucose as a cosubstrate. MS analysis identified the protein substrate as the mammalian elongation factor (EF)1A.\n                    <jats:italic>Legionella<\/jats:italic>\n                    glucosyltransferase modifies its eukaryotic protein substrate at serine-53, which is located in the GTPase domain of the EF. Glucosylation of EF1A results in inhibition of eukaryotic protein synthesis and death of target cells. Our findings show a mode of inhibition of protein synthesis by microbial pathogens and offer a perspective for understanding of the host-pathogen interaction of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.0601562103","type":"journal-article","created":{"date-parts":[[2006,10,26]],"date-time":"2006-10-26T20:50:35Z","timestamp":1161895835000},"page":"16953-16958","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":161,"title":["<i>Legionella pneumophila<\/i>\n                    glucosyltransferase inhibits host elongation factor 1A"],"prefix":"10.1073","volume":"103","author":[{"given":"Yury","family":"Belyi","sequence":"first","affiliation":[{"name":"Gamaleya Research Institute, Ulitsa Gamalei 18, Moscow 123098, Russia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricarda","family":"Niggeweg","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bastian","family":"Opitz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Infectious and Pulmonary Diseases, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Vogelsgesang","sequence":"additional","affiliation":[{"name":"Institute of Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs-Universit\u00e4t Freiburg, Albertstrasse 25, D-79104 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Hippenstiel","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Infectious and Pulmonary Diseases, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Wilm","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Aktories","sequence":"additional","affiliation":[{"name":"Institute of Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs-Universit\u00e4t Freiburg, Albertstrasse 25, D-79104 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,11,7]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.506-526.2002"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1997.00440360129015"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.6.2108"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.99.6.1936"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(88)90079-4"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.4.1319"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/15687"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.2.862-870.1999"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.7.3029-3034.1998"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2000.00076.x"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.7.4040-4051.2004"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.950-958.1998"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01316.x"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01092.x"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2004.07.001"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2005.09.005"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.1.181-186.2003"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10254-004-0033-5"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/375500a0"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.31.19566"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.06.071"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.73.030403.080419"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.1.403-413.2005"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201022200"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb979"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.74.061903.155440"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(03)00066-5"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)00122-2"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.10.12.8903506"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1062023"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(96)00123-2"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi972592c"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi034529v"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)98937-4"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)47093-2"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001005200"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2004.12.007"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1463"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00150-7"},{"key":"e_1_3_4_40_2","first-page":"27","volume":"175","author":"Wilson BA","year":"1992","unstructured":"BA Wilson, RJ Collier Curr Top Microbiol Immunol 175, 27\u201342 (1992).","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.1.240-246.1991"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5339-5349.2005"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac950914h"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300486"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3628-3634.2001"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0601562103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T15:53:33Z","timestamp":1649778813000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0601562103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,7]]},"references-count":46,"journal-issue":{"issue":"45","published-print":{"date-parts":[[2006,11,7]]}},"alternative-id":["10.1073\/pnas.0601562103"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0601562103","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1048260.500174","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11,7]]},"assertion":[{"value":"2006-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-11-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}