{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:10:11Z","timestamp":1791159011424,"version":"4.1.0"},"reference-count":24,"publisher":"National Academy of Sciences","issue":"45","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,11,7]]},"abstract":"<jats:p>\n                    CD36 and LIMPII analog 1, CLA-1, and its splicing variant, CLA-2 (SR-BI and SR-BII in rodents), are human high density lipoprotein receptors with an identical extracellular domain which binds a spectrum of ligands including bacterial cell wall components. In this study, CLA-1- and CLA-2-stably transfected HeLa and HEK293 cells demonstrated several-fold increases in the uptake of various bacteria over mock-transfected cells. All bacteria tested, including both Gram-negatives (\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    K12, K1 and\n                    <jats:italic>Salmonella typhimurium<\/jats:italic>\n                    ) and Gram-positives (\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    and\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    ), demonstrated various degrees of lower uptake in control cells. This result is consistent with the presence of high-density lipoprotein-receptor-independent bacterial uptake that is enhanced by CLA-1\/CLA-2 overexpression. Bacterial lipopolysaccharides, lipoteichoic acid, and synthetic amphipathic helical peptides (L-37pA and D-37pA) competed with\n                    <jats:italic>E. coli<\/jats:italic>\n                    K12 for CLA-1 and CLA-2 binding. Transmission electron microscopy and confocal microscopy revealed cytosolic accumulation of bacteria in CLA-1\/CLA-2-overexpressing HeLa cells. The antibiotic protection assay confirmed that\n                    <jats:italic>E. coli<\/jats:italic>\n                    K12 was able to survive and replicate intracellularly in CLA-1- and CLA-2-overexpressing HeLa, but both L-37pA and D-37pA prevented\n                    <jats:italic>E. coli<\/jats:italic>\n                    K12 invasion. Peritoneal macrophages isolated from SR-BI\/BII-knockout mice demonstrated a 30% decrease in bacterial uptake when compared with macrophages from normal mice. Knockout macrophages were also characterized by decreased bacterial cytosolic invasion, ubiquitination, and proteasome mobilization while retaining bacterial lysosomal accumulation. These results indicate that, by facilitating bacterial adhesion and cytosolic invasion, CLA-1 and CLA-2 may play an important role in infection and sepsis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0602126103","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T20:45:14Z","timestamp":1161981914000},"page":"16888-16893","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":62,"title":["CLA-1 and its splicing variant CLA-2 mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells"],"prefix":"10.1073","volume":"103","author":[{"given":"Tatyana G.","family":"Vishnyakova","sequence":"first","affiliation":[{"name":"*National Heart, Lung, and Blood Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Kurlander","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Clinical Center,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander V.","family":"Bocharov","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Clinical Center,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina N.","family":"Baranova","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Clinical Center,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Chen","sequence":"additional","affiliation":[{"name":"*National Heart, Lung, and Blood Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mones S.","family":"Abu-Asab","sequence":"additional","affiliation":[{"name":"National Cancer Institute, and the"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Tsokos","sequence":"additional","affiliation":[{"name":"National Cancer Institute, and the"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Malide","sequence":"additional","affiliation":[{"name":"Light Microscopy Core Facility, National Heart, Lung, and Blood Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Basso","sequence":"additional","affiliation":[{"name":"*National Heart, Lung, and Blood Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Remaley","sequence":"additional","affiliation":[{"name":"*National Heart, Lung, and Blood Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyorgy","family":"Csako","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Clinical Center,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas L.","family":"Eggerman","sequence":"additional","affiliation":[{"name":"Division of Diabetes, Endocrinology, and Metabolic Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy P.","family":"Patterson","sequence":"additional","affiliation":[{"name":"*National Heart, Lung, and Blood Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,11,7]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200114011"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-200406000-00008"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509042200"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02373.x"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)30277-7"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.28.17551"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0011633"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411555200"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405009200"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00100.x"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211032200"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M314264200"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1116006"},{"key":"e_1_3_4_14_2","author":"Bocharov AV","year":"2004","unstructured":"AV Bocharov, IN Baranova, G Csako, TL Eggerman, AP Patterson, AT Remaley, TG Vishnyakova International patent WO, 2004\/041179 A2. (2004).","journal-title":"International patent WO"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20278"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002411200"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/09680519050110061001"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(01)00084-8"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.04.033"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.5.2471"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(81)90007-1"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305289200"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411600200"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.8.4523-4530.2000"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0602126103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T15:52:58Z","timestamp":1649778778000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0602126103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,7]]},"references-count":24,"journal-issue":{"issue":"45","published-print":{"date-parts":[[2006,11,7]]}},"alternative-id":["10.1073\/pnas.0602126103"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0602126103","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11,7]]},"assertion":[{"value":"2006-03-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-11-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}