{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:35:00Z","timestamp":1791160500159,"version":"4.1.0"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"36","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,9,5]]},"abstract":"<jats:p>\n                    The pathogenic species of\n                    <jats:italic>Yersinia<\/jats:italic>\n                    contain the transcriptional regulator RovA. In\n                    <jats:italic>Yersinia pseudotuberculosis<\/jats:italic>\n                    and\n                    <jats:italic>Yersinia enterocolitica<\/jats:italic>\n                    , RovA regulates expression of the invasion factor invasin (\n                    <jats:italic>inv<\/jats:italic>\n                    ), which mediates translocation across the intestinal epithelium. A\n                    <jats:italic>Y<\/jats:italic>\n                    . enterocolitica rov\n                    <jats:italic>A<\/jats:italic>\n                    mutant has a significant decrease in virulence by LD\n                    <jats:sub>50<\/jats:sub>\n                    analysis and an altered rate of dissemination compared with either wild type or an\n                    <jats:italic>inv<\/jats:italic>\n                    mutant, suggesting that RovA regulates multiple virulence factors. Here, we show the involvement of RovA in the virulence of\n                    <jats:italic>Yersinia pestis<\/jats:italic>\n                    , which naturally lacks a functional\n                    <jats:italic>inv<\/jats:italic>\n                    gene. A\n                    <jats:italic>Y<\/jats:italic>\n                    . pestis \u0394rov\n                    <jats:italic>A<\/jats:italic>\n                    mutant is attenuated \u224880-fold by LD\n                    <jats:sub>50<\/jats:sub>\n                    and is defective in dissemination\/colonization of spleens and lungs after s.c. inoculation. However, the \u0394rov\n                    <jats:italic>A<\/jats:italic>\n                    mutant is only slightly attenuated when given via an intranasal or i.p. route, indicating a more important role for RovA in bubonic plague than pneumonic plague or systemic infection. Microarray analysis was used to define the RovA regulon. The\n                    <jats:italic>psa<\/jats:italic>\n                    locus was among the most highly down-regulated loci in the \u0394rov\n                    <jats:italic>A<\/jats:italic>\n                    mutant. A \u0394\n                    <jats:italic>psaA<\/jats:italic>\n                    mutant had a significant dissemination defect after s.c. infection but only slight attenuation by the pneumonic-disease model, closely mimicking the virulence defect seen with the \u0394rov\n                    <jats:italic>A<\/jats:italic>\n                    mutant. DNA-binding studies revealed that RovA specifically interacts with the\n                    <jats:italic>psaE<\/jats:italic>\n                    and\n                    <jats:italic>psaA<\/jats:italic>\n                    promoter regions, indicating a direct role for RovA in regulating this locus. Thus, RovA appears to be a global transcription factor in\n                    <jats:italic>Y<\/jats:italic>\n                    . pestis and, through its regulatory influence on genes such as\n                    <jats:italic>psaEFABC<\/jats:italic>\n                    , contributes to the virulence of\n                    <jats:italic>Y<\/jats:italic>\n                    . pestis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0603456103","type":"journal-article","created":{"date-parts":[[2006,8,28]],"date-time":"2006-08-28T22:10:40Z","timestamp":1156803040000},"page":"13514-13519","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":145,"title":["RovA, a global regulator of\n                    <i>Yersinia pestis<\/i>\n                    , specifically required for bubonic plague"],"prefix":"10.1073","volume":"103","author":[{"given":"Jason S.","family":"Cathelyn","sequence":"first","affiliation":[{"name":"Departments of *Molecular Microbiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth D.","family":"Crosby","sequence":"additional","affiliation":[{"name":"Pediatrics, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wyndham W.","family":"Lathem","sequence":"additional","affiliation":[{"name":"Departments of *Molecular Microbiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William E.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Departments of *Molecular Microbiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia L.","family":"Miller","sequence":"additional","affiliation":[{"name":"Departments of *Molecular Microbiology and"},{"name":"Genome Sequencing Center, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,9,5]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"423","volume":"13","author":"Bacot A. 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