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Rat spermatogonial stem cells were collected from immature testis and transplanted into the seminiferous tubules of busulfan-treated nude mouse testis. Using rat spermatids or spermatozoa that developed in xenogeneic surrogate mice, rat offspring were born from fresh and cryopreserved donor cells after microinsemination with rat oocytes. These offspring were fertile and had a normal imprinting pattern. The xenogeneic offspring production by interspecies germ cell transplantation and\n                    <jats:italic>in vitro<\/jats:italic>\n                    microinsemination will become a powerful tool in animal transgenesis and species conservation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0604205103","type":"journal-article","created":{"date-parts":[[2006,8,30]],"date-time":"2006-08-30T20:44:37Z","timestamp":1156970677000},"page":"13624-13628","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":60,"title":["Rats produced by interspecies spermatogonial transplantation in mice and\n                    <i>in vitro<\/i>\n                    microinsemination"],"prefix":"10.1073","volume":"103","author":[{"given":"Takashi","family":"Shinohara","sequence":"first","affiliation":[{"name":"*Department of Molecular Genetics and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megumi","family":"Kato","sequence":"additional","affiliation":[{"name":"National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Takehashi","sequence":"additional","affiliation":[{"name":"*Department of Molecular Genetics and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyoung","family":"Lee","sequence":"additional","affiliation":[{"name":"*Department of Molecular Genetics and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichiro","family":"Chuma","sequence":"additional","affiliation":[{"name":"Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Nakatsuji","sequence":"additional","affiliation":[{"name":"Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mito","family":"Kanatsu-Shinohara","sequence":"additional","affiliation":[{"name":"*Department of Molecular Genetics and"},{"name":"Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masumi","family":"Hirabayashi","sequence":"additional","affiliation":[{"name":"National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,9,12]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1002\/j.1939-4640.2000.tb03408.x","volume":"21","author":"de Rooij DG","year":"2000","unstructured":"DG de Rooij, LD Russell J Androl 21, 776\u2013798 (2000).","journal-title":"J Androl"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1093\/oso\/9780195062694.003.0012","volume-title":"Cell and Molecular Biology of the Testis","author":"Meistrich ML","year":"1993","unstructured":"ML Meistrich, MEAB van Beek Cell and Molecular Biology of the Testis, eds C Desjardins, LL Ewing (Oxford Univ. 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