{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:07:01Z","timestamp":1787234821602,"version":"build-2736575974"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,2,6]]},"abstract":"<jats:p>\n                    Human uterine endometrium exhibits unique properties of cyclical regeneration and remodeling throughout reproductive life and also is subject to endometriosis through ectopic implantation of retrogradely shed endometrial fragments during menstruation. Here we show that functional endometrium can be regenerated from singly dispersed human endometrial cells transplanted beneath the kidney capsule of NOD\/SCID\/\u03b3\n                    <jats:sub arrange=\"stack\">c<\/jats:sub>\n                    <jats:sup arrange=\"stack\">null<\/jats:sup>\n                    immunodeficient mice. In addition to the endometrium-like structure, hormone-dependent changes, including proliferation, differentiation, and tissue breakdown and shedding (menstruation), can be reproduced in the reconstructed endometrium, the blood to which is supplied predominantly by human vessels invading into the mouse kidney parenchyma. Furthermore, the hormone-dependent behavior of the endometrium regenerated from lentivirally engineered endometrial cells expressing a variant luciferase can be assessed noninvasively and quantitatively by\n                    <jats:italic>in vivo<\/jats:italic>\n                    bioluminescence imaging. These results indicate that singly dispersed endometrial cells have potential applications for tissue reconstitution, angiogenesis, and human\u2013mouse chimeric vessel formation, providing implications for mechanisms underlying the physiological endometrial regeneration during the menstrual cycle and the establishment of endometriotic lesions. This animal system can be applied as the unique model of endometriosis or for other various types of neoplastic diseases with the capacity of noninvasive and real-time evaluation of the effect of therapeutic agents and gene targeting when the relevant cells are transplanted beneath the kidney capsule.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0604310104","type":"journal-article","created":{"date-parts":[[2007,2,2]],"date-time":"2007-02-02T13:37:31Z","timestamp":1170423451000},"page":"1925-1930","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":130,"title":["Noninvasive and real-time assessment of reconstructed functional human endometrium in NOD\/SCID\/\u03b3\n                    <sub>c<\/sub>\n                    <sup>null<\/sup>\n                    immunodeficient mice"],"prefix":"10.1073","volume":"104","author":[{"given":"Hirotaka","family":"Masuda","sequence":"first","affiliation":[{"name":"Departments of *Obstetrics and Gynecology,"},{"name":"Physiology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Departments of *Obstetrics and Gynecology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emi","family":"Hiratsu","sequence":"additional","affiliation":[{"name":"Physiology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Yamane","sequence":"additional","affiliation":[{"name":"Physiology, and"},{"name":"Orthopedic Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Iwanami","sequence":"additional","affiliation":[{"name":"Physiology, and"},{"name":"Orthopedic Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Departments of *Obstetrics and Gynecology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Ono","sequence":"additional","affiliation":[{"name":"Departments of *Obstetrics and Gynecology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Subteam for Manipulation of Cell Fate, BioResource Center, RIKEN Tsukuba Institute, Ibaraki 305-0074, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotaka James","family":"Okano","sequence":"additional","affiliation":[{"name":"Physiology, and"},{"name":"Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Saitama 332-0012, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamoru","family":"Ito","sequence":"additional","affiliation":[{"name":"**Central Institute for Experimental Animals, Kanagawa 216-0001, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norikazu","family":"Tamaoki","sequence":"additional","affiliation":[{"name":"**Central Institute for Experimental Animals, Kanagawa 216-0001, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuji","family":"Nomura","sequence":"additional","affiliation":[{"name":"**Central Institute for Experimental Animals, Kanagawa 216-0001, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Okano","sequence":"additional","affiliation":[{"name":"Physiology, and"},{"name":"Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Saitama 332-0012, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumi","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Physiology, and"},{"name":"Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Saitama 332-0012, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasunori","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Departments of *Obstetrics and Gynecology,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2007,2,6]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(01)00379-4"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.6.1686"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)17403-5"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dml026"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2001-12-0207"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002-021888"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1042\/bst0280191"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/15.2.284"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700900108"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)61745-2"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)00191-X"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.4.111901.093336"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1432"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.21.12044"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-4082fje"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0102-87"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.2032388"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1991.tb37849.x"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0411"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)00601-4"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/16.8.1736"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2002.tb02793.x"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002-021912"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.03.034"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.5.1612.405a13_1612_1621"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/349329a0"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-41320"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0557fje"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1230217"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64251-4"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.6.6933"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211715200"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.10.8150-8157.1998"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0604310104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T14:52:38Z","timestamp":1649775158000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0604310104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2,6]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,2,6]]}},"alternative-id":["10.1073\/pnas.0604310104"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0604310104","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1063873.518775","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1063873.516812","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1063873.518627","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2,6]]},"assertion":[{"value":"2006-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-02-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}