{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:36:01Z","timestamp":1781109361162,"version":"3.54.1"},"reference-count":44,"publisher":"National Academy of Sciences","issue":"35","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,8,29]]},"abstract":"<jats:p>\n                    Accumulating evidence indicates that plasma membrane (PM) microdomains and the subjacent \u201cjunctional\u201d sarcoplasmic\/endoplasmic reticulum (jS\/ER) constitute specialized Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling complexes in many cell types. We examined the possibility that some Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals arising in the junctional space between the PM and jS\/ER may represent cross-talk between the PM and jS\/ER. The Ca\n                    <jats:sup>2+<\/jats:sup>\n                    sensor protein, GCaMP2, was targeted to different PM domains by constructing genes for fusion proteins with either the \u03b11 or \u03b12 isoform of the Na\n                    <jats:sup>+<\/jats:sup>\n                    pump catalytic (\u03b1) subunit. These fusion proteins were expressed in primary cultured mouse brain astrocytes and arterial smooth muscle cells. Immunocytochemistry demonstrated that \u03b12(f)GCaMP2, like native Na\n                    <jats:sup>+<\/jats:sup>\n                    pumps with \u03b12-subunits, sorted to PM domains that colocalized with subjacent S\/ER; \u03b11(f)GCaMP2, like Na\n                    <jats:sup>+<\/jats:sup>\n                    pumps with \u03b11-subunits, was more uniformly distributed. The GCaMP2 moieties in both constructs were tethered just beneath the PM. Both constructs detected global Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals evoked by serotonin (in arterial smooth muscle cells) and ATP, and by store-operated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channel-mediated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    entry after S\/ER unloading with cyclopiazonic acid (in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -free medium). When cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    diffusion was markedly restricted with EGTA, however, only \u03b12(f)GCaMP2 detected the local, store-operated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channel-mediated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    entry signal. Thus, \u03b11 Na\n                    <jats:sup>+<\/jats:sup>\n                    pumps are apparently excluded from the PM microdomains occupied by \u03b12 Na\n                    <jats:sup>2+<\/jats:sup>\n                    pumps. The jS\/ER and adjacent PM may communicate by Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals that are confined to the tiny junctional space between the two membranes. Similar methods may be useful for studying localized Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals in other subPM microdomains and signals associated with other organelles.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0605757103","type":"journal-article","created":{"date-parts":[[2006,8,21]],"date-time":"2006-08-21T20:39:52Z","timestamp":1156192792000},"page":"13232-13237","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":80,"title":["Local subplasma membrane Ca\n                    <sup>2+<\/sup>\n                    signals detected by a tethered Ca\n                    <sup>2+<\/sup>\n                    sensor"],"prefix":"10.1073","volume":"103","author":[{"given":"Moo Yeol","family":"Lee","sequence":"first","affiliation":[{"name":"Departments of *Physiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Song","sequence":"additional","affiliation":[{"name":"Departments of *Physiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Nakai","sequence":"additional","affiliation":[{"name":"Laboratory for Memory and Learning, RIKEN Brain Science Institute, Hirosawa, Wako, Saitama 351-0198, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masamichi","family":"Ohkura","sequence":"additional","affiliation":[{"name":"First Department of Pharmacology, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, Yoshino, Nobeoka, Miyazaki 882-8508, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael I.","family":"Kotlikoff","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14835"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen P.","family":"Kinsey","sequence":"additional","affiliation":[{"name":"Departments of *Physiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera A.","family":"Golovina","sequence":"additional","affiliation":[{"name":"Departments of *Physiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mordecai P.","family":"Blaustein","sequence":"additional","affiliation":[{"name":"Departments of *Physiology and"},{"name":"Medicine, University of Maryland School of Medicine, Baltimore, MD 21201;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,8,29]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.2.H679"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2003.10.013"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2003.10.011"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.085035"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(02)75325-3"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01176186"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.70.2.338"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01970000"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310365200"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/242244a0"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.278.1.C163"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00383.2002"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.7.1575"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-198600088-00023"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016800"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509378103"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32359-1"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.275.5.F633"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-02-00381.1991"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00389.2003"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.091801"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.5.1800"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1997.tb52310.x"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M507450200"},{"key":"e_1_3_4_25_2","first-page":"F240","volume":"266","author":"Tumlin J. 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