{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:49:13Z","timestamp":1781106553766,"version":"3.54.1"},"reference-count":56,"publisher":"National Academy of Sciences","issue":"36","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,9,5]]},"abstract":"<jats:p>\n                    To investigate the functions of circadian neurons, we added two strategies to the standard\n                    <jats:italic>Drosophila<\/jats:italic>\n                    behavioral genetics repertoire. The first was to express a polyglutamine-expanded neurotoxic protein (MJDtr78Q; MJD, Machado\u2013Joseph disease) in the major\n                    <jats:italic>timeless<\/jats:italic>\n                    (\n                    <jats:italic>tim<\/jats:italic>\n                    )-expressing cells of the adult brain. These Tim-MJD flies were viable, in contrast to the use of cell-death gene expression for\n                    <jats:italic>tim<\/jats:italic>\n                    neuron inactivation. Moreover, they were more arrhythmic than flies expressing other neurotoxins and had low but detectable\n                    <jats:italic>tim<\/jats:italic>\n                    mRNA levels. The second extended standard microarray technology from fly heads to dissected fly brains. By combining the two approaches, we identified a population of Tim-MJD-affected mRNAs. Some had been previously identified as sex-specific and relevant to courtship, including mRNAs localized to brain-proximal fat-body tissue and brain courtship centers. Finally, we found a decrease in the number of neurons that expressed male-specific forms of the\n                    <jats:italic>fruitless<\/jats:italic>\n                    protein in the laterodorsal region of the brain. The decrease was not a consequence of toxic protein expression within these specialized cells but a likely effect of communication with neighboring TIM-expressing neurons. The data suggest a functional interaction between adjacent circadian and mating circuits within the fly brain, as well as an interaction between circadian circuits and brain-proximal fat body.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0605962103","type":"journal-article","created":{"date-parts":[[2006,8,28]],"date-time":"2006-08-28T22:10:40Z","timestamp":1156803040000},"page":"13537-13542","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":29,"title":["Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in\n                    <i>Drosophila<\/i>"],"prefix":"10.1073","volume":"103","author":[{"given":"Sebastian","family":"Kadener","sequence":"first","affiliation":[{"name":"*Department of Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Villella","sequence":"additional","affiliation":[{"name":"*Department of Biology 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and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey C.","family":"Hall","sequence":"additional","affiliation":[{"name":"*Department of Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Rosbash","sequence":"additional","affiliation":[{"name":"*Department of Biology and"},{"name":"Howard Hughes Medical Institute and National Center for Behavioral Genomics, Brandeis University, Waltham, MA 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