{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:27:50Z","timestamp":1789648070459,"version":"4.0.0"},"reference-count":38,"publisher":"National Academy of Sciences","issue":"44","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,10,31]]},"abstract":"<jats:p>\n                    <jats:italic>Mixl1<\/jats:italic>\n                    , the sole murine homologue of the\n                    <jats:italic>Xenopus Mix\/Bix<\/jats:italic>\n                    family of homeobox transcription factors, is essential for the patterning of axial mesendodermal structures during early embryogenesis. Gene targeting and overexpression studies have implicated\n                    <jats:italic>Mixl1<\/jats:italic>\n                    as a regulator of hematopoiesis arising in differentiating embryonic stem cells. To assess the role of\n                    <jats:italic>Mixl1<\/jats:italic>\n                    in the regulation of adult hematopoiesis, we overexpressed\n                    <jats:italic>Mixl1<\/jats:italic>\n                    in murine bone marrow using a retroviral transduction\/transplantation model. Enforced expression of\n                    <jats:italic>Mixl1<\/jats:italic>\n                    profoundly perturbed hematopoietic lineage commitment and differentiation, giving rise to abnormal myeloid progenitors and impairing erythroid and lymphoid differentiation. Moreover, all mice reconstituted with\n                    <jats:italic>Mixl1<\/jats:italic>\n                    -transduced bone marrow developed fatal, transplantable acute myeloid leukemia with a mean latency period of 200 days. These observations establish a link between enforced\n                    <jats:italic>Mixl1<\/jats:italic>\n                    expression and leukemogenesis in the mouse.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0607776103","type":"journal-article","created":{"date-parts":[[2006,10,24]],"date-time":"2006-10-24T10:19:21Z","timestamp":1161685161000},"page":"16460-16465","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":12,"title":["Enforced expression of the homeobox gene\n                    <i>Mixl1<\/i>\n                    impairs hematopoietic differentiation and results in acute myeloid leukemia"],"prefix":"10.1073","volume":"103","author":[{"given":"Stefan","family":"Glaser","sequence":"first","affiliation":[{"name":"*The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald","family":"Metcalf","sequence":"additional","affiliation":[{"name":"*The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wu","sequence":"additional","affiliation":[{"name":"*The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam H.","family":"Hart","sequence":"additional","affiliation":[{"name":"*The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ladina","family":"DiRago","sequence":"additional","affiliation":[{"name":"*The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Mifsud","sequence":"additional","affiliation":[{"name":"*The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"D'Amico","sequence":"additional","affiliation":[{"name":"*The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Dagger","sequence":"additional","affiliation":[{"name":"Telethon Institute for Child Health Research, 100 Roberts Road, Subiaco, Western Australia 6008, Australia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Campo","sequence":"additional","affiliation":[{"name":"Telethon Institute for Child Health Research, 100 Roberts Road, Subiaco, Western Australia 6008, Australia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela C.","family":"Chan","sequence":"additional","affiliation":[{"name":"Telethon Institute for Child Health Research, 100 Roberts Road, Subiaco, Western Australia 6008, Australia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Izon","sequence":"additional","affiliation":[{"name":"Telethon Institute for Child Health Research, 100 Roberts Road, Subiaco, Western Australia 6008, Australia; and"},{"name":"St. Vincent's Institute, 9 Princes Street, Fitzroy, Victoria 3065, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorraine","family":"Robb","sequence":"additional","affiliation":[{"name":"*The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,10,31]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.20-5-364"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00135-5"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0177(2000)9999:9999<::AID-DVDY1070>3.0.CO;2-O"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90335-8"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.14.2577"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.8.1769"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5373.91"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.19.4193"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.10.1279"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.21.4901"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.13.2371"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.20.3997"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.2.167"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/382357a0"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10263"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01657"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.06.044"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.15.3597"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(95)80071-9"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-10-4120"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V100.1.89"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.2.488"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/35004599"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030107"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V100.1.238"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.21.8398"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80238-1"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.1.495"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.13.3714"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1583"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5439.531"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10198"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403816"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0305555101"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.9.2181"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.10.3780"},{"key":"e_1_3_4_37_2","first-page":"9.9.1","volume-title":"Current Protocols in Molecular Biology","author":"Pear WS","year":"1996","unstructured":"WS Pear, C Cepko Current Protocols in Molecular Biology, eds FM Ausubel, R Brent, RE Kingston, DD Moore, JG Seidman, JA Smith, K Struhl (Wiley, New York), pp. 9.9.1\u20139.9.16 (1996)."},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.14.5006-5018.2002"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0607776103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T15:24:42Z","timestamp":1649777082000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0607776103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,31]]},"references-count":38,"journal-issue":{"issue":"44","published-print":{"date-parts":[[2006,10,31]]}},"alternative-id":["10.1073\/pnas.0607776103"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0607776103","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10,31]]},"assertion":[{"value":"2006-10-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}