{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T08:04:38Z","timestamp":1791014678344,"version":"4.1.0"},"reference-count":44,"publisher":"National Academy of Sciences","issue":"45","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2006,11,7]]},"abstract":"\n Previous biochemical studies of the\n BLM<\/jats:italic>\n gene product have shown its ability in conjunction with topoisomerase III\u03b1 to resolve double Holliday structures through a process called \u201cdissolution.\u201d This process could prevent crossing over during repair of double-strand breaks. We report an analysis of the\n Drosophila<\/jats:italic>\n BLM<\/jats:italic>\n gene,\n DmBlm<\/jats:italic>\n , in the repair of double-strand breaks in the premeiotic germ line of\n Drosophila<\/jats:italic>\n males. With a repair reporter construct, Rr3, and other genetic tools, we show that\n DmBlm<\/jats:italic>\n mutants are defective for homologous repair but show a compensating increase in single-strand annealing. Increases of 40- to 50-fold in crossing over and flanking deletions also were seen. Perhaps most significantly, the template used for homologous repair in\n DmBlm<\/jats:italic>\n mutants is itself subject to deletions and complex rearrangements. These template disruptions are indicative of failure to resolve double Holliday junctions. These findings, along with the demonstration that a weak allele of topoisomerase III\u03b1 has some of the same defects as\n DmBlm<\/jats:italic>\n , support the dissolution model. Finally, an analysis of\n DmBlm<\/jats:italic>\n mutants in conjunction with\n mus81<\/jats:italic>\n or\n spnA<\/jats:italic>\n (\n Rad51<\/jats:italic>\n ) reveals a second function of\n BLM<\/jats:italic>\n distinct from the repair of induced double-strand breaks and possibly related to maintenance of replication forks.\n <\/jats:p>","DOI":"10.1073\/pnas.0607904103","type":"journal-article","created":{"date-parts":[[2006,10,30]],"date-time":"2006-10-30T20:48:28Z","timestamp":1162241308000},"page":"16840-16845","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":28,"title":["Template disruptions and failure of double Holliday junction dissolution during double-strand break repair in\n Drosophila BLM<\/i>\n mutants"],"prefix":"10.1073","volume":"103","author":[{"given":"Dena","family":"Johnson-Schlitz","sequence":"first","affiliation":[{"name":"Genetics Department, University of Wisconsin, Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William R.","family":"Engels","sequence":"additional","affiliation":[{"name":"Genetics Department, University of Wisconsin, Madison, WI 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2006,11,7]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.66.4.630-670.2002"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.2741\/1938"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206679"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.12.3.1292-1303.1992"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.5.2164"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030491"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.11.4508"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90105-1"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki712"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.49.30611"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02253"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0508295103"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600740"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604873103"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601828103"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505211200"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200110009"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604268200"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200405128"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403000101"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077198"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.10.059"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl258"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.105.050138"},{"key":"e_1_3_4_25_2","author":"Preston CR","year":"2006","unstructured":"CR Preston, CC Flores, WR Engels Curr Biol, 2006).","journal-title":"Curr Biol"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.291.5513.2600"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/151.3.1027"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/97.3-4.607"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406157101"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411337200"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj068"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.23.8758-8766.2000"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00536-0"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00593-1"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/157.1.103"},{"key":"e_1_3_4_36_2","unstructured":"M Ashburner Drosophila A Laboratory Handbook. 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