{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:19:12Z","timestamp":1791242352875,"version":"4.1.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"20","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,5,15]]},"abstract":"<jats:p>\n                    Manganese toxicity is a major problem for plant growth in acidic soils, but cellular mechanisms that facilitate growth in such conditions have not been clearly delineated. Established mechanisms that counter metal toxicity in plants involve chelation and cytoplasmic export of the metal across the plasma or vacuolar membranes out of the cell or sequestered into a large organelle, respectively. We report here that expression of the\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    and poplar MTP11 cation diffusion facilitators in a manganese-hypersensitive yeast mutant restores manganese tolerance to wild-type levels. Microsomes from yeast expressing\n                    <jats:italic>AtMTP11<\/jats:italic>\n                    exhibit enhanced manganese uptake. In accord with a presumed function of MTP11 in manganese tolerance,\n                    <jats:italic>Arabidopsis mtp11<\/jats:italic>\n                    mutants are hypersensitive to elevated levels of manganese, whereas plants overexpressing\n                    <jats:italic>MTP11<\/jats:italic>\n                    are hypertolerant. In contrast, sensitivity to manganese deficiency is slightly decreased in mutants and increased in overexpressing lines. Promoter-GUS studies showed that\n                    <jats:italic>AtMTP11<\/jats:italic>\n                    is most highly expressed in root tips, shoot margins, and hydathodes, but not in epidermal cells and trichomes, which are generally associated with manganese accumulation. Surprisingly, imaging of MTP11\u2013EYFP fusions demonstrated that MTP11 localizes neither to the plasma membrane nor to the vacuole, but to a punctate endomembrane compartment that largely coincides with the distribution of the trans-Golgi marker sialyl transferase. Golgi-based manganese accumulation might therefore result in manganese tolerance through vesicular trafficking and exocytosis. In accord with this proposal,\n                    <jats:italic>Arabidopsis mtp11<\/jats:italic>\n                    mutants exhibit enhanced manganese concentrations in shoots and roots. We propose that Golgi-mediated exocytosis comprises a conserved mechanism for heavy metal tolerance in plants.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0609507104","type":"journal-article","created":{"date-parts":[[2007,5,9]],"date-time":"2007-05-09T20:53:32Z","timestamp":1178744012000},"page":"8532-8537","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":264,"title":["A secretory pathway-localized cation diffusion facilitator confers plant manganese tolerance"],"prefix":"10.1073","volume":"104","author":[{"given":"Edgar","family":"Peiter","sequence":"first","affiliation":[{"name":"*Department of Biology, University of York, P.O. Box 373, York YO10 5YW, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Montanini","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Mixte de Recherche, Institut National de la Recherche Agronomique\/Universit\u00e9 Henri Poincar\u00e9, 1136 Interactions Arbres-Microorganismes, Nancy-Universit\u00e9, 54506 Vandoeuvre-les-Nancy, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Gobert","sequence":"additional","affiliation":[{"name":"*Department of Biology, University of York, P.O. Box 373, York YO10 5YW, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pai","family":"Pedas","sequence":"additional","affiliation":[{"name":"Plant and Soil Science Laboratory, Department of Agricultural Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren","family":"Husted","sequence":"additional","affiliation":[{"name":"Plant and Soil Science Laboratory, Department of Agricultural Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans J. M.","family":"Maathuis","sequence":"additional","affiliation":[{"name":"*Department of Biology, University of York, P.O. Box 373, York YO10 5YW, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Blaudez","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Mixte de Recherche, Institut National de la Recherche Agronomique\/Universit\u00e9 Henri Poincar\u00e9, 1136 Interactions Arbres-Microorganismes, Nancy-Universit\u00e9, 54506 Vandoeuvre-les-Nancy, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Chalot","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Mixte de Recherche, Institut National de la Recherche Agronomique\/Universit\u00e9 Henri Poincar\u00e9, 1136 Interactions Arbres-Microorganismes, Nancy-Universit\u00e9, 54506 Vandoeuvre-les-Nancy, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale","family":"Sanders","sequence":"additional","affiliation":[{"name":"*Department of Biology, University of York, P.O. 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